AMB  >> Vol. 4 No. 3 (September 2015)

    Progress in the Study of the Biological Activities and Extraction and Purification of Edible Mushroom Polysaccharides

  • 全文下载: PDF(431KB) HTML   XML   PP.45-54   DOI: 10.12677/AMB.2015.43007  
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食用菌多糖生物学活性Edible Mushroom Polysaccharide Biological Activity



Edible Mushroom polysaccharides exhibit varied bio-activities such as immunomodulation, anti-cancer, prevention and treatment of cardiovascular diseases, antimicrobial and antiviral effects. Multiple steps of extraction, separation and purification are needed to get high yield and high purity of edible mushroom polysaccharides. The biological activities of edible mushroom polysaccharides are significantly affected by their sequences, structures, and quaternary configurations, and thus systematic characterization of the structures and configurations is indespensible for understanding the biological functions of the edible mushroom polysaccharides. This article reviews the main methods of isolation, purification and structural characterization of the edible mushroom polysaccharide, as well as some of the important biological activities.

汪治云. 食用菌多糖的生物学活性及提取纯化研究进展[J]. 微生物前沿, 2015, 4(3): 45-54.


[1] Gogavekar, S.S., Rokade, S.A., Ranveer, R.C., Ghosh, J.S., Kalyani, D.C. and Sahoo, A.K. (2014) Important nutritional constituents, flavour components, antioxidant and antibacterial properties of Pleurotus sajor-caju. Journal of Food Science and Technology, 51, 1483-1491.
[2] Pons, M., Dauphin, B., La Guerche, S., Pons, A., Lavigne-Cruege, V., Shinkaruk, S., Bunner, D., Richard, T., Monti, J.P. and Darriet, P. (2011) Identification of impact odorants contributing to fresh mushroom off-flavor in wines: Incidence of their reactivity with nitrogen compounds on the decrease of the olfactory defect. Journal of Agricultural and Food Chemistry, 59, 3264-3272.
[3] Tsai, S.Y., Tsai, H.L. and Mau, J.L. (2008) Non-volatile taste components of Agaricus blazei, Agrocybe cylindracea and Boletus edulis. Food Chemistry, 107, 977-983.
[4] Chang, S.T. and Wasser, S.P. (2012) The role of culi-nary-medicinal mushrooms on human welfare with a pyramid model for human health. International Journal of Medicinal Mushrooms, 14, 95-134.
[5] Bohn, J.A. and BeMiller, J.N. (1995) (1→3)-β-D-glucans as biological response modifiers: A review of structure- functional activity relationships. Carbohydrate Polymers, 28, 3-14.
[6] Dore, C., Azevedo, T.C.G., de Souza, M.C.R., Rego, L.A., de Dantas, J.C.M., Silva, F.R.F., Rocha, H.A.O., Basela, I.G. and Leite, E.L. (2007) Antiinflammatory, antioxidant and cytotoxic actions of beta-glucan-rich extract from Geastrum saecatum mushroom. International Immunopharmacology, 7, 1160-1169.
[7] Yue, L., Cui, H., Li, C., Lin, Y., Sun, Y., Niu, Y., Wen, X. and Liu, J. (2012) A polysaccharide from Agaricus blazei attenuates tumor cell adhesion via inhibiting E-selectin expression. Carbohydrate Polymers, 88, 1326-1333.
[8] Lee, K.H., Cho, C.H. and Rhee, K.H. (2011) Synergic anti-tumor activity of gamma-irradiated exo-polysaccharide from submerged culture of Grifola frondosa. Journal of Medical Plants Research, 5, 2378-2386.
[9] Hu, Q., Wang, H. and Ng, T.B. (2012) Isolation and purification of polysaccharides with anti-tumor activity from Pholiota adiposa (Batsch) P. Kumm. (higher basidiomycetes). International Journal of Medicinal Mushrooms, 14, 271- 284.
[10] Wang, G.B., Dong, L.L., Zhang, Y.Y., Ji, Y.Y., Xiang, W.H. and Zhao, M. (2012) Polysaccharides from Phellinus linteus inhibit cell growth and invasion and induce apoptosis in HepG2 human hepatocellular carcinoma cells. Biologia, 67, 247-254.
[11] Chihara, G., Hamuro, J., Maeda, Y.Y., Shiio, T., Suga, T., Takasuka, N. and Sasaki, T. (1987) Antitumor and metastasis-inhibitory activities of lentinan as an immunomodulator: An overview. Cancer Detection and Prevention, 1, 423- 443.
[12] Higashi, D., Seki, K., Ishibashi, Y., Egawa, Y., Koga, M., Sasaki, T., Hirano, K., Mikami, K., Futami, K., Maekawa, T., Sudo, M. (2012) The effect of lentinan combination therapy for unresectable advanced gastric cancer. Anticancer Research, 32, 2365-2368.
[13] Ng, M.L. and Yap, A.T. (2002) Inhibition of human colon carcinoma development by lentinan from shiitake mushrooms (Lentinus edodes). The Journal of Alternative Complementary Medicine, 8, 581-589.
[14] Ma, J.W., Qiao, Z.Y. and Xiang, X. (2011) Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities. Carbohydrate Polymers, 84, 1061-1068.
[15] Shimizu, C., Kihara, M., Aoe, S., Araki, S., Ito, K., Hayashi, K., Watari, J., Sakata, Y. and Ikegami, S. (2008) Effect of high beta-glucan barley on serum cholesterol concentrations and visceral fat area in japanese men—A randomized, double-blinded, placebo-controlled trial. Plant Foods for Human Nutrition, 63, 21-25.
[16] Naumann, E., van Rees, A.B., Onning, G., Oste, R., Wydra, M. and Mensink, R.P. (2006) Beta-glucan incorporated into a fruit drink effectively lowers serum LDL-cholesterol concentrations. The American Journal of Clinical Nutrition, 83, 601-605.
[17] Drozdowski, L.A., Reimer, R.A., Temelli, F., Bell, R.C., Vasanthan, T. and Thomson, A.B.R. (2010) β-glucan extracts inhibit the in vitro intestinal uptake of long-chain fatty acids and cholesterol and down-regulate genes involved in lipogenesis and lipid transport in rats. The Journal of Nutritional Biochemistry, 21, 695-701.
[18] Gordon, M., Guralnik, M., Kaneko, Y., Mimura, T., Goodgame, J., DeMarzo, C., Pierce, D., Baker, M. and Lang, W. (1995) A phase II controlled study of a combination of the immune modulator, lentinan, with didanosine (ddi) in HIV patients with CD4 cells of 200 - 500/mm3. Journal of Medicine, 26, 193-207.
[19] Drandarska, I., Kussovski, V., Nikolaeva, S. and Markova, N. (2005) Combined immunomodulating effects of BCG and lentinan after intranasal application in guinea pigs. International Immunopharmacology, 5, 795-803.
[20] 周萍, 安东, 王朝川, 刘雪梅, 李新胜 (2011) 食用菌复合多糖的抗氧化活性研究. 中国食用菌, 30, 42-44.
[21] Barone Lumaga, R., Azzali, D., Fogliano, V., Scalfi, L. and Vitaglione, P. (2012) Sugar and dietary fibre composition influence, by different hormonal response, the satiating capacity of a fruit-based and a β-glucan-enriched beverage. Food & Function, 3, 67-75.
[22] Ye, M., Chen, W.X., Qiu, T., Yuan, R.Y., Ye ,Y.-W. and Cai, J.M. (2012) Structural characterisation and anti-ageing activity of extracellular polysaccharide from a strain of Lachnum sp. Food Chemistry, 132, 338-343.
[23] Velebny, S., Hrckova, G. and Kogan, G. (2008) Impact of treatment with praziquantel, silymarin and/or β-glucan on pathophysiological markers of liver damage and fibrosis in mice infected with Mesocestoides vogae (Cestoda) tetrathyridia. Journal of Helminthology, 82, 211-219.
[24] Da Silva, M.D.C., Fukuda, E.K., Vasconcelos, A.F.D., Dekker, R.F.H., Matias, A.C., Monteiro, N.K., Cardoso, M.S., Barbosa, A.M., Silveira, J.L.M. and Sassaki, G.L. (2008) Structural characterization of the cell wall D-glucans isolated from the mycelium of Botryosphaeria rhodina MAMB-05. Carbohydrate Research, 343, 793-798.
[25] Muller, A., Ensley, H., Pretus, H., McNamee, R., Jones, E., McLaughlin, E., Chandley, W., Browder, W., Lowman, D. and Williams, D. (1997) The application of various protic acids in the extraction of (1→3)-β-D-glucan from Saccharomyces cerevisiae. Carbohydrate Research, 299, 203-208.
[26] Lo, T.C.T., Tsao, H.H., Wang, A.Y. and Chang, C.A. (2007) Pressurized water extraction of polysaccharides as secondary metabolites from Lentinula edodes. Journal of Agricultural and Food Chemistry, 55, 4196-4201.
[27] Santoyo, S., Plaza, M., Jaime, L., Ibanez, E., Reglero, G. and Senorans, F.J. (2010) Pressurized liquid extraction as an alternative process to obtain antiviral agents from the edible microalga Chlorella vulgaris. Journal of Agricultural and Food Chemistry, 58, 8522-8527.
[28] Fu, Y.J., Liu, W., Zu, Y.G., Shi, X.G., Liu, Z.G., Schwarz, G. and Efferth, T. (2009) Breaking the spores of the fungus Ganoderma lucidum by supercritical CO2. Food Chemistry, 112, 71-76.
[29] Tian, Y.T., Zeng, H.L., Xu, Z.B., Zheng, B.D., Lin, Y.X., Gan, C.J. and Lo, Y.M. (2012) Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus). Carbohydrate Polymers, 88, 522-529.
[30] Zhang, Z., Lv, G., Pan, H., Shi, L. and Fan, L. (2011) Optimization of the microwave-assisted extraction process for polysaccharides in himematsutake (Agaricus blazei Murrill) and evaluation of their antioxidant activities. Food Science and Technology Research, 17, 461-470.
[31] Song, J.F., Li, D.J. and Liu, C.Q. (2009) Response surface analysis of microwave-assisted extraction of polysaccharides from cultured Cordyceps militaris. Journal of Chemical Technology and Biotechnology, 84, 1669-1673.
[32] Dong, J.Z., Wang, Z.C. and Wang, Y. (2011) Rapid extraction of polysaccharides from fruits of Lycium barbarum L. Journal of Food Biochemistry, 35, 1047-1057.
[33] Huang, S.Q. and Ning, Z.X. (2010) Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity. International Journal of Biological Macromolecules, 47, 336-341.
[34] Palacios, I., Lozano, M., Moro, C., D’Arrigo, M., Rostagno, M.A., Martínez, J.A., García-Lafuente, A., Guillamón, E. and Villares, A. (2011) Antioxidant properties of phenolic compounds occurring in edible mushrooms. Food Chemistry, 128, 674-678.
[35] Park, H.G., Shim, Y.Y., Choi, S.O. and Park, W.M. (2009) New method development for nanoparticle extraction of water-soluble β-(1→3)-D-glucan from edible mushrooms, Sparassis crispa and Phellinus linteus. Journal of Agricultural and Food Chemistry, 57, 2147-2154.
[36] Ragaee, S.M., Wood, P.J., Wang, Q., Tosh, S.M., Brummer, Y. and Huang, X. (2008) Isolation, fractionation, and structural characteristics of alkali-extractable beta-glucan from rye whole meal. Cereal Chemistry, 85, 289-294.
[37] Li, W., Cui, S.W. and Kakuda, Y. (2006) Extraction, fractionation, structural and physical characterization of wheat β-D-glucans. Carbohydrate Polymers, 63, 408-416.
[38] Bao, X.F., Fang, J.N. and Li, X.Y. (2001) Structural characterization and immunomodulating activity of a complex glucan from spores of Ganoderma lucidum. Bioscience, Biotechnology, and Biochemistry, 65, 2384-2391.
[39] Ye, L.B., Zhang, J.S., Yang, Y., Zhou, S.A., Liu, Y.F., Tang, Q.L., Du, X.J., Chen, H. and Pan, Y.J. (2009) Structural characterisation of a heteropolysaccharide by NMR spectra. Food Chemistry, 112, 962-966.
[40] Masuko, T., Minami, A., Iwasaki, N., Majima, T., Nishimura, S. and Lee, Y.C. (2005) Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Analytical Biochemistry, 339, 69-72.
[41] Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426-428.
[42] Singleton, V.L. and Rossi Jr., J.A. (1965) Colorimetry of total phenolics with phosphomolybdic- phophotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
[43] Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
[44] Johansson, L., Virkki, L., Anttila, H., Esselstrom, H., Tuomainen, P. and Sontag-Strohm, T. (2006) Hydrolysis of beta- glucan. Food Chemistry, 97, 71-79.
[45] Blakeney, A.B., Harris, P.J., Henry, R.J. and Stone, B.A. (1983) A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohydrate Research, 113, 291-299.
[46] Ciucanu, I. (2006) Per-O-methylation reaction for structural analysis of carbohydrates by mass spectrometry. Analytica Chimica Acta, 576, 147-155.
[47] Wang, L., Zhang, H.B., Zhang, X.Y. and Chen, Z.X. (2008) Purification and identification of a novel heteropolysaccharide RBPS2a with anti-complementary activity from defatted rice bran. Food Chemistry, 110, 150-155.
[48] Calonje, M., García Mendoza, C., Perez Cabo, A. and No-vaes-Ledieu, M. (1996) New contributions to the wall polysaccharide structure of the vegetative mycelium and fruit body cell walls of Agaricus bisporus. Microbiología, 12, 599-606.
[49] Lee, J.S., Kwon, J.S., Won, D.P., Lee, K.E., Shin, W.C. and Hong, E.K. (2010) Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris. Carbohydrate Polymers, 82, 982-988.
[50] Yoshioka, Y., Uehara, N. and Saito, H. (1992) Conforma-tion-dependent change in antitumor-activity of linear and branched (1→3)-beta-D-glucans on the basis of conformational elucidation by C-13 Nuclear Magnetic Resonance spectroscopy. Chemical & Pharmaceutical Bulletin, 40, 1221-1226.
[51] Kulicke, W.M., Lettau, A.I. and Thielking, H. (1997) Correlation between immunological activity, molar mass, and molecular structure of different (1→3)-beta-D-glucans. Carbohydrate Research, 297, 135-143.
[52] Jelsma, J. and Kreger, D.R. (1975) Ultrastructural observations on (1→3)-beta-D-glucan from fungal cell-walls. Carbohydrate Research, 43, 200-203.
[53] 叶立斌, 张劲松, 潘迎捷 (2007) 食用菌多糖结构解析中的核磁共振技术. 食用菌学报, 24, 68-75.