ACM  >> Vol. 2 No. 4 (December 2012)

    Expression of Imprinted Genes Igf2r and RB-1 in the Endometrium of Infertile Women with Metabolic Syndrome

  • 全文下载: PDF(335KB) HTML   XML   PP.27-31   DOI: 10.12677/ACM.2012.24006  
  • 下载量: 2,256  浏览量: 9,340  


王俊豪,阮 钰:北京大学深圳医院生殖医学科,深圳;汕头大学医学院,汕头;
李 蓉:北京大学深圳医院生殖医学科,深圳

印迹基因Igf2rRB-1代谢综合征 Imprinted Genes; Igf2r; RB-1; Metabolic Syndrome


目的:研究印记基因Igf2rRB-1在代谢综合征(MS)不孕患者子宫内膜的表达。方法:选择20117月至201112月因不孕症来我院生殖中心就诊的MS患者11例作为实验组,代谢正常女性15例作为对照组,收集分泌期的子宫内膜组织,采用实时定量聚合酶链反应方法(RT-PCR)测定子宫内膜组织Igf2rRB-1的表达水平。结果Igf2rRB-1MS患者和代谢正常妇女子宫内膜中均有表达,与正常女性相比较,MS患者子宫内膜Igf2r表达水平下降(P = 0.0051)RB-1表达水平升高(P = 0.0364),具有显著性差异。结论MS不孕患者子宫内膜组织印记基因Igf2r表达水平明显下降,RB-1表达水平明显升高,两者可能影响MS患者的子宫内膜容受性。

Objective: To investigate expression of imprinted genes Igf2r, RB-1 inthe endometrium of infertile women with metabolic syndrome (MS). Methods: From Jul. 2011 to Dec.2011 inour center, 11 infecund women with metabolic abnormalities were experimental group, a matched group of 15 normal women was choosed. Collected their prolife rative phase endometrial tissue, using real-time quantitative polymerase chain reaction (RT-PCR) to measure the ex- pressions of Igf2r and Rb-1 inthe endometrium. Results: Igf2r and RB-1 were both expressed in the endometrium of MS and normal women. Compared with the normal women, the expression of Igf2r in the endometrium of MS was decreased (P = 0.0051), while the expression of RB-1 was incresed (P = 0.0364), significant differences were observed between experimental group and matched group. Conclusions: The expression of imprinted gene Igf2r in the endo- metrium of infertile women with MS was decreased significantly, while the expression of RB-1 was increased, the 2 imprinted genes may impair the function of endometrium of MS.


王俊豪, 阮钰, 李蓉. 印记基因Igf2r和RB-1在代谢综合征不孕患者子宫内膜表达水平的研究[J]. 临床医学进展, 2012, 2(4): 27-31.


[1] Y. Linne. Effects of obesity on women reproduction and com- plications during pregnancy. Obesity Reviews, 2004, 5(3): 137- 143.
[2] F. Hall, A. Neubert. Obesity and pregnancy. Obstetrical & Gynecological Survey, 2005, 60(4): 253-260.
[3] M. Mitchell, D. T. Armstrong, R. L. Robker, et al. Adipokines: Implications for female fertility and obesity. Reproduction, 2005, 130(5): 583-597.
[4] J. Bellver, M. A. Melo, E. Bosch, et al. Obesity and poor repro- ductive outcome: The potential role of the endometrium. Fertility and Sterility, 2007, 88( 2): 446-451
[5] A. Naumova, C. Sapienza. The genetics of retinoblastoma, re- visited. American Journal of Human Genetics, 1994, 54(2): 264- 273.
[6] R. G. Edwards. Genetics, epigenetics and gene silencing in dif- ferentiating mammalian embryos. Reproductive BioMedicine On- line, 2006, 13(5): 732-753.
[7] P. E. Szabó, J. R. Mann. Allele-specific expression and total ex- pression levels of imprinted genes during early mouse develop- ment: Implications for imprinting mechanisms. Genes & Development, 1995, 9(24): 3097-108
[8] D. Lonmann. Imprinting of the RB1 gene and parent-of-origin effects in retinoblastoma. Medizinische Genetik, 2010, 2: 429- 433.
[9] L. E. Young, K. Fernandes, T. G. McEvoy, S. C. Butterwith, C. G. Gutierrez, C. Carolan, P. J. Broadben, J. J. Robinson, I. Wilmut and K. D. Sinclair. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nature Genetics, 2001, 27(2): 153-154
[10] S. Xie, Z. Wang, M. Okano, M. Nogami, Y. Li, W. W. He, K. Okumura and E. Li. Cloning, expression and chromosome loca- tions of the human DNMT3 gene family. Gene, 1999, 236(1): 87- 95.
[11] D. Lucifero, C. Mertineit, H. J. Clarke, T. H. Bestor and J. M. Trasler. Methylation dynamics of imprinted genes in mouse germ cells. Genomics, 2002, 79(4): 530-538.
[12] D. Lonmann. Imprinting of the RB1 gene and parent-of-origin effects in retinoblastoma. Medizinische Genetik, 2010, 2: 429- 433.
[13] P. Bischof, A. Meissue and A. Campana. Mechanisms of endo- metrial control of trophoblast invasion. Journal of Reproductive Fertile Supple, 2000, 55: 65-71.