基本情况

王来曦，美国马里兰大学化学与生物化学系教授。

 

研究领域

生物有机化学、多糖化学、多糖生物学、酶学、免疫学

 

教育背景

1997年至2000年 博士后，美国麻省理工学院生物系

1994年至1997年 博士后，美国约翰霍普金斯大学生物系

1984年至1991年 理学博士，中国科学院上海有机化学研究所


工作经历

2015-present  Professor, Department of Chemistry and Biochemistry, University of Maryland College Park, College Park, MD

2009-present  Professor, Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, MD

2009-present  Professor, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD

2007-2009  Associate Professor, Department of Biochemistry & Molecular Biology,

University of Maryland School of Medicine, Baltimore, MD

2005-2009  Associate Professor, Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, Baltimore, MD

2000-2005  Tenure-Track Assistant Professor, Institute of Human Virology, University of Maryland, Baltimore, MD

1997-2000  Research Associate, Massachusetts Institute of Technology (MIT), Cambridge, MA

1994-1997  Postdoctoral Fellow, Johns Hopkins University, Baltimore, MD

1991-1993  Faculty Member (1991-1992, Assistant Professor; 1992-1993, Associate Professor), Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences

 

获奖与荣誉

2017  Chair, the 2017 Gordon Research Conferences (GRC) on Carbohydrates

2016  Chair, Division of Carbohydrate Chemistry, the American Chemical Society

2016  Member, the U.S. Advisory Committee for International Carbohydrate Symposia (ICS)

2015  Vice Chair, the 2015 Gordon Research Conferences (GRC) on Carbohydrates

2014  Melville L. Wolfrom Award in Carbohydrate Chemistry, the American Chemical Society

2014  Elected AAAS Fellow

2009  Inducted to the Johns Hopkins University Society of Scholars

2004  Young Investigator Award from the Division of Carbohydrate Chemistry, the American Chemical Society

1988-1991  Pre-doctoral Fellowship, RIKEN Institute, Japan

 

论文发表

  1. Li C, Zhu S, Ma C, and Wang LX, “Designer 1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins”, J. Am. Chem. Soc., accepted (2017)
  2. Cai H, Orwenyo J, Giddens JP, Yang Y, Zhang, R, LaBranche CC, Montefiori DC, Wang LX, “Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses”, Cell Chemical Biology, accepted (2017)
  3. Yang Q, Wang LX, “Chemoenzymatic Glycan Remodeling of Natural and Recombinant Glycoproteins”, Methods Enzymol., 597, 265-281 (2017)
  4. Tong X, Li T, Orwenyo J, Toonstra C, Wang LX, “One-pot enzymatic glycan remodeling of a therapeutic monoclonal antibody by endoglycosidase S (Endo-S) from Streptococcus pyogenes”, Bioorg. Med. Chem., accepted (2017)
  5. Yang Q, Zhang R, Cai H, Wang LX. “Revisiting the substrate specificity of mammalian α1, 6-fucosyltransferase (FUT8) reveals that it catalyzes core fucosylation of N-glycans lacking α1,3-arm GlcNAc”, J. Biol. Chem., 292, 14796-14803 (2017)
  6. Tang F, Wang LX, Huang W. “Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates”, Nature Protocols, 12(8): 1702-1721 (2017)
  7. Yang M, Huang J, Simon R, Wang LX, MacKerell AD Jr. “Conformational Heterogeneity of the HIV Envelope Glycan Shield”, Scientific Reports, 7(1): 4435 (2017)
  8. Orwenyo J, Cai H, Giddens J, Amin MN, Toonstra C, Wang LX. “Systematic synthesis and binding study of HIV V3 glycopeptides reveal the fine epitopes of several broadly neutralizing antibodies”, ACS Chem. Biol., 12, 1566-1575 (2017)
  9. Cai H, Orwenyo J, Guenaga J, Giddens J, Toonstra C, Wyatt RT, Wang LX, “Synthetic multivalent V3 glycopeptides display enhanced recognition by glycan-dependent HIV-1 broadly neutralizing antibodies”, Chem. Commun. 53, 5453-5456 (2017)
  10. Yang Q, An Y, Zhu S, Zhang R, Loke CM, Cipollo JF, Wang LX, “Glycan Remodeling of Human Erythropoietin (EPO) Through Combined Mammalian Cell Engineering and Chemoenzymatic Transglycosylation”, ACS Chem. Biol., 12, 1665-1673 (2017)
  11. Feng C, Li J, Snyder G, Huang W, Goldblum SE, Chen WH, Wang LX, McClane BA, Cross AS, “Antibody against Microbial Neuraminidases Recognizes Human Sialidase 3 (NEU3): The Neuraminidase/Sialidase Superfamily Revisited”, MBio, 8, pii: e00078-17 (2017)
  12. Li, T., DiLillo, D., Bournazos, S., Giddens, J. P., Ravetch, J. V., Wang, L. X., “Modulating IgG effector functions by Fc glycan engineering”, Proc. Natl. Acad. Sci. USA, 114, 3485-3490 (2017)
  13. Freund NT, Wang H, Scharf L, Nogueira L, Horwitz JA, Bar-On Y, Golijanin J, Sievers SA, Sok D, Cai H, Cesar Lorenzi JC, Halper-Stromberg A, Toth I, Piechocka-Trocha A, Gristick HB, van Gils MJ, Sanders RW, Wang LX, Seaman MS, Burton DR, Gazumyan A, Walker BD, West AP Jr, Bjorkman PJ, Nussenzweig MC, “Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller”, Science Translational Medicine, 9, eaal2144 (2017)
  14. Yamaguchi, T., Amin, M.N., Toonstra, C., Wang, L.X., “Chemoenzymatic synthesis and receptor binding of mannose-6-phosphate (M6P)-containing glycoprotein ligands reveal unusual structural requirements for M6P receptor recognition”, J. Am. Chem. Soc., 138, 12472-85 (2016). 15. Toonstra, C., Amin, M.N., Wang, L.X., “Site-selective chemoenzymatic glycosylation of an HIV-1 polypeptide antigen with two distinct N-glycans via an orthogonal protecting group strategy”, J. Org. Chem., 81, 6176-6185 (2016)
  15. Hsiao, PY, Kalin, JH, Sun, IH, Amin, MN, Lo, YC, Chiang, MJ, Giddens, J, Sysa-Shah, P, Gabrielson, K, Wang, LX, Powell, JD, Cole, PA, “An Fc-small molecule conjugate for targeted inhibition of the adenosine 2A receptor”, ChemBioChem, 17, 1951-1960 (2016)
  16. Li, T., Tong, X., Yang, Q., Giddens, J.P., Wang, L.X., “Glycosynthase mutants of endoglycosidase S2 show potent transglycosylation activity and remarkably relaxed substrate specificity for antibody glycosylation remodeling”, J. Biol. Chem., 291, 16508-16518 (2016)
  17. Yang, Q., Wang, L.X., “Mammalian α-1, 6-fucosyltransferase (FUT8) is the sole enzyme responsible for the N-acetylglucosaminyltransferase I-independent core fucosylation of high-mannose N-glycans”, J. Biol. Chem., 291, 9356-9370 (2016)
  18. Giddens, J.P., Lomino, J.V., Amin, M.N., Wang, L.X., “Endo-F3 glycosynthase mutants enable chemoenzymatic synthesis of core fucosylated tri-antennary complex-type glycopeptides and glycoproteins”, J. Biol. Chem., 291, 11064-11071 (2016)
  19. Liu, R., Giddens, J., McClung, C.M., Magnelli, P.E., Wang, L.X., Guthrie, E.P., “Evaluation of a glycoengineered monoclonal antibody via LC-MS analysis in combination with multiple enzymatic digestion”, MAbs, 8, 340-6 (2016)
  20. Quast, I., Keller, C.W., Maurer, M.A., Giddens, J.P., Tackenberg, B., Wang, L.X., Münz, C., Nimmerjahn, F., Dalakas, M.C., Lünemann, J.D., “Sialylation of IgG Fc domain impairs complement-dependent cytotoxicities”, J. Clin. Invest., 125, 4160-70 (2015)
  21. Feng C, Ghosh A, Amin MN, Bachvaroff TR, Tasumi S, Pasek M, Banerjee A, Shridhar S, Wang LX, Bianchet MA, Vasta GR. “Galectin CvGal2 from the Eastern Oyster (Crassostrea virginica) Displays Unique Specificity for ABH Blood Group Oligosaccharides and Differentially Recognizes Sympatric Perkinsus Species”. Biochemistry, 54, 4711-30 (2015)
  22. Giddens JP, Wang LX. “Chemoenzymatic Glyco-engineering of Monoclonal Antibodies”. Methods Mol Biol., 1321, 375-87 (2015)
  23. Wang D, Tang J, Tang J, Wang LX, “Targeting N-glycan cryptic sugar moieties for broad-spectrum virus neutralization: progress in identifying conserved molecular targets in viruses of distinct phylogenetic origins”. Molecules. 20, 4610-22 (2015)
  24. Nita-Lazar M, Banerjee A, Feng C, Amin MN, Frieman MB, Chen WH, Cross AS, Wang LX, Vasta GR. “Desialylation of airway epithelial cells during influenza virus infection enhances pneumococcal adhesion via galectin binding”, Mol Immunol., 65, 1-16 (2015)
  25. Ahmed, A.A, Giddens, J.P., Pincetic, A., Lomino, J.V., Ravetch, J.V., Wang, L.X., Bjorkman, P.J., “Structural characterization of anti-inflammatory Immunoglobulin G Fc proteins”, J. Mol. Biol., 426, 3166-79 (2014)
  26. Trastoy, B., Lomino, J.V., Pierce, B.G., Carter, L.G., Gunther, S., Giddens, J.P., Snyder, G.A., Weiss, T.M., Weng, Z., Wang, L.X., Sundberg, E.J., “Crystal structure of Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies”, Proc. Natl. Acad. Sci. USA, 111, 6714-6719 (2014)
  27. Smith, E.L., Giddens, J.P., Iavarone, A.T., Godula, K., Wang, L.X., Bertozzi, C.R., “Chemoenzymatic Fc glycosylation via engineered aldehyde tags”, Bioconjugate Chem., 25, 788-795 (2014)
  28. Chiang, M.J., Holbert, M.A., Kalin, J.H., Ahn, Y.H., Giddens, J, Amin, M.N., Taylor, M.S., Collins, S.L., Chan-Li, Y, Waickman, A, Hsiao, P.Y., Bolduc, D, Leahy, D.J., Horton, M.R., Wang, L.X., Powell, J.D., Cole, P.A., “An Fc domain protein-small molecule conjugate as an enhanced immunomodulator. J. Am. Chem. Soc., 136, 3370-3373 (2014)
  29. Wang, L.X., Amin, M.N., “Chemical and chemoenzymatic synthesis of glycoproteins for deciphering functions” (Invited Review), Chemistry & Biology, 21, 51-66 (2014)
  30. Wang, L.X., “Synthetic carbohydrate antigens for HIV vaccine design” (Invited Review), Current Opinion in Chemical Biology, 17, 997–1005 (2013)
  31. Wang, L.X., Davis, B.G., “Realizing the promise of chemical glycobiology” (Invited Review), Chemical Science, 4, 3381-3394 (2013)
  32. Amin, M.N., McLellan, J.S., Huang, W., Orwenyo, J., Burton, D.R., Koff, W.C., Kwong, P.D., Wang, L.X., “Synthetic glycopeptides reveal the glycan specificity of HIV-neutralizing antibodies”, Nature Chem. Biol., 9, 521-526 (2013)
  33. Pancera, M., Shahzad-ul-Hussan, S., Doria-Rose, N. A., McLellan, J.S., Dai, K., Loesgen, S., Staupe, S.P., Yang, Y., Haynes, B. F., Amin, M.N., Wang, L. X., Burton, D. R., Koff, W.C., Nabel, G.J., Mascola, J.R., Bewley, C.A., Kwong, P.D., “Structural basis for diverse N-glycan recognition and enhanced HIV-1 neutralization by V1/V2-directed antibodies”, Nature Struct. Mol. Biol., 20, 804-813 (2013)
  34. Orwenyo, J., Huang, W., Wang, L.X., “Chemoenzymatic synthesis and lectin recognition of a selectively fluorinated glycoprotein”, Bioorg. Med. Chem., 21, 4768-77 (2013)
  35. Lomino, J.V., Naegeli, A., Orwenyo, J., Amin, M.N., Aebi, M., Wang, L.X., “A two-step enzymatic glycosylation of polypeptides with complex N-glycans”, Bioorg. Med. Chem., 21, 2262-70 (2013)
  36. Wang, H., Huang, W., Orwenyo, J., Banerjee, A., Vasta, G.R., Wang, L.X., “Design and synthesis of glycoprotein-based multivalent glyco-ligands for influenza hemagglutinin and human galectin-3”. Bioorg. Med. Chem., 21, 2037-44 (2013)
  37. Heredia, A, Davis, C.E., Reitz, M.S., Le, N.M., Wainberg, M.A., Foulke, J.S., Wang, L.X., Redfield, R.R.,” Targeting of the purine biosynthetic host cell pathway enhances the activity of tenofovir against sensitive drug-resistant HIV-1”, J. Infect. Dis., 208, 2085-94 (2013)
  38. Feng, C., Ghosh, A., Amin, M.N., Giomarelli, B., Shridhar, S., Banerjee, A., Fernández-Robledo, J.A., Bianchet, M.A., Wang, L.X., Wilson, I.B., Vasta, G.R., “The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface”, J. Biol. Chem., 288, 24394-409 (2013)
  39. Huang, W., Giddens, J., Fan, S.Q., Toonstra, C., Wang, L.X., “Chemoenzymatic glycoengineering of intact IgG antibodies for gain of functions”, J. Am. Chem. Soc., 134, 12308−12318 (2012)
  40. Fan, S., Huang, W., Wang, L. X. “Remarkable transglycosylation activity of glycosynthase mutants of Endo-D, an endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae”, J. Biol. Chem., 287, 11272–11281 (2012)
  41. Lillehoj, E.P., Hyun, S.W., Feng, C., Zhang, L., Liu, A., Guang, W., Nguyen, C., Luzina, I.G., Atamas, S.P., Passaniti, A., Twaddell, W.S., Puché, A.C., Wang, L.X., Cross, A.S., Goldblum, S.E. “NEU1 sialidase expressed in human airway epithelia regulates epidermal growth factor receptor (EGFR) and MUC1 protein signaling”, J. Biol. Chem., 287, 8214-8231 (2012)
  42. Wang, L. X., Lomino, J. V. “Emerging technologies for making glycan-defined glycoproteins” (Invited Review), ACS Chemical Biology, 7, 110-122 (2011)
  43. McLellan, J., Pancera, M., Carrico, C., Gorman, J., Julien, J. P., Khayat, R., Louder, R., Pejchal, R., Sastry, M., Dai, K., O’Dell, S., Patel, N., Shahzad-ul-Hussan, S., Yang, Y., Zhang, B., Zhou, T., Zhu, J., Boyington, J. C., Chuang, G. Y., Diwanji, D., Georgiev, I., Kwon, Y. D., Lee, D., Louder, M. K., Moquin, S., Schmidt, S. D., Yang, Z. Y., Bonsignori, M., Crump, J. A., Kapiga, S. H., Sam, N. E., Haynes, B. F., Burton, D. R., Koff, W. C., Walker, L. M., Phogat, S., Wyatt, R., Orwenyo, J., Wang, L. X., Arthos, J., Bewley, C. A., Mascola, J. R., Nabel, G. J., Schief, W. R., Ward, A. B., Wilson, I. A., Kwong, P. D., “Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9”, Nature, 480, 336-343 (2011)
  44. Zou, G., Ochiai, H., Huang, W., Yang, Q., Li, C., Wang, L. X., “Chemoenzymatic synthesis and Fc receptor binding of homogeneous glycoforms of antibody Fc domain. Presence of a bisecting sugar moiety enhances the affinity of Fc to FcIIIa receptor”, J. Am. Chem. Soc., 133, 18975-18991 (2011)
  45. Amin, M. N., Huang, W., Mizanur, R. M., Wang, L. X., “Convergent synthesis of homogeneous Glc1Man9GlcNAc2-protein and derivatives as ligands of molecular chaperones in protein quality control”, J. Am. Chem. Soc., 133, 14404–14417 (2011)
  46. Huang, W., Li, J., Wang, L. X., “Unusual transglycosylation activity of Flavobacterium meningosepticum endoglycosidases enables convergent chemoenzymatic synthesis of core fucosylated complex N-glycopeptides”, ChemBioChem, 12, 932-941 (2011)