小麦麦谷蛋白亚基基因密码子用法特性分析

Characterization Analysis of Codon Usage of Glutenin Subunits Genes in Wheat

摘要: 该文运用CodonW1.4.2和SPSS16.0软件对13个来源于小麦麦谷蛋白亚基基因的密码子用法特性进行分析。结果显示，除1Dy10基因ENC值小于40外，其余亚基基因ENC值均介于40~50之间；GC3含量均在0.38~0.45之间；1Bx14基因偏向于使用A或T结尾的密码子，其余12个亚基基因偏向于使用G或C结尾的密码子；1Dy10与其余12个亚基基因间距离系数较大，然而各组染色体麦谷蛋白x-型或y-型亚基基因间距离系数较小。结果表明，小麦麦谷蛋白亚基基因具有较强的密码子使用偏爱性，各基因密码子用法上具有相似性，每一种氨基酸均存在1~2个偏爱密码子。在密码子用法差异上表现为，除1By9和1Dy10之外，无论是A染色体组、B染色体组，还是D染色体组，各组染色体上麦谷蛋白x-型或y-型亚基基因间密码子使用频率差异最小。这一分析结果对小麦HMW-GS功能基因资源的高效利用和LMW-GS新基因的克隆具有一定的指导意义。

Abstract: The codon characters of 13 high molecular weight glutenin subunits genes from wheat were analyzed by software CodonW1.4.2 and SPSS16.0. The results showed that ENC of HMW-GS genes ranges between 40 and 50, but 1Dy10 gene’ is less than 40; GC3 contents are between 0.38 and 0.45; 1Bx14 gene prefers to use A or T at the end of codon, and the remaining 12 genes prefer to use G or C ending codons; the Euclidean distance coefficient is minimum between the alleles. The results indicated that the wheat HMW-GS genes have strong codon usage bias; the codon usage is similar; there are bias codons for each amino acid. Considering the codon usage differences, except 1By9 and 1Dy10, the difference of codon use frequency is minimum between x- or y-type subunit genes of glutenin in each chromosome group. The results have certain significance in the efficient use of HMW-GS genetic resources and the cloning of new LMW-GS genes.