摘要:
本文研究表面活性剂Tx-100介质中诱惑红对水溶性碲化镉量子点(CdTe QDs)荧光猝灭效应。建立Tx-100增敏CdTe QDs荧光猝灭法测定诱惑红(Allura red)新方法。结果表明：Tx-100介质中，Allura red对CdTe QDs荧光猝灭值增大，Allura red浓度在0.6~60.0 µg/mL范围内与CdTe QDs荧光猝灭值呈良好线性关系，其线性回归方程为ΔF=12.86c + 77.36 (c:µg/mL)，线性相关系数为0.9939，检出限为0.35µg/mL(检出限定义为LOD = 3 σ/k，其中，σ是标准偏差，k是标准曲线的斜率)。本文初步探讨了增敏机制和在不同介质中的荧光量子产率。该法测定实际样品中Allura red，结果令人满意。
Abstract:
The fluorescence quenching effect of Allura red on the water-soluble CdTe quantum dots (CdTe QDs) in surfactant Tx-100 medium was investigated. Based on the Tx-100 sensitization CdTe QDs fluorescence quenching, a novel method for the determination of Allura red was proposed. Under the optimal conditions, the relationship of Allura red concentration in the 0.6 - 60.0 µg/mL range and CdTe QDs fluorescence quenching value was linear. The linear regression equation and the linear correlation were ΔF = 12.86c-77.36 (c:µg/mL) and 0.9939, respectively. The limit of detection was found to be 0.35 µg/mL (the definition of limit of detection was LOD = 3σ/k, where σ was the standard deviation and k was the slope of the standard curve). The sensitized mechanism and fluorescence quantum yield in different media were discussed preliminarily. The method has been applied to the determination of Allura red in samples with satisfactory results.