水提取杜仲干粉及活性成分工艺研究

Study on the Technology of Water Extraction of Eucommia Dry Powder and Active Ingredients

摘要: 以杜仲干粉为原料，采用单因素实验方法探讨料液比(1:5~1:30 g∙mL⁻¹)、提取时间(0.5~3.0 h)和提取温度(60~100℃)对杜仲干粉总固形物得率及活性成分提取率的影响，并确定单因素实验最佳提取工艺为：料液比1:20，提取时间1.5 h，提取温度100℃。此条件下，总固形物得率为1.86%，其中活性成分绿原酸得率为0.0164%，桃叶珊瑚苷得率为0.0317%，京尼平苷得率为0.0063%，松脂醇二葡萄糖苷得率为0.0696%。水提取杜仲干粉方法简单，无环境污染和溶剂回收问题，本文为杜仲干粉活性成分提取和开发利用提供的实验依据。

Abstract: Using Eucommia dry powder as raw material, the single-factor experimental method was used to investigate the ratio of material to liquid (1:5 - 1:30 g∙mL⁻¹), extraction time (0.5 - 3.0 h) and extraction temperature (60˚C - 100˚C) to Eucommia dry powder. The effect of total solid yield and active ingredient extraction rate was established. The optimal extraction process for single factor experiment was as follows: material to liquid ratio 1:20, extraction time 1.5 h, extraction temperature 100˚C. The total solid yield was 1.86%; chlorogenic acid yield was 0.0164%; aucubin yield was 0.0317%; geniposide yield was 0.0063%; pinoresinol diglucoside yield was 0.0696% under the optimum extraction processes for single-factor experiment. The method of water extraction of Eucommia dry powder is simple, and hasn’t the problems of environmental pollution and solvent recovery. This paper provides an experimental basis for the extraction and development of active ingredients of Eucommia dry powder.