分子生物技术在污泥微生物群落多样性研究中的应用—污泥微生物群落研究
Application in Activated Sludge Microbial Community Diversity Used Molecular Biological Technology—Study of Sludge Microbial Community Diversity
摘要: 以活性污泥法为主体的生化好氧处理工艺是目前应用最广泛的污水生物处理技术。随着分子生物学技术的发展，人们对活性污泥微生物菌群的复杂性和多样性的认识逐步深入，大量依靠传统方法未能检测出，但却在活性污泥中起关键作用的微生物逐渐被发现。这些现代分子生物技术以FISH技术、DGGE/TGGE技术、T-RFLP技术、RAPD技术、AFLP技术、PCR-SSCP技术等为代表。通过对这些技术的综述分析，揭示其技术特点和应用发展方向，为探索研究活性污泥的微生物群落多样性提供指导意义。
Abstract: Biochemical aerobic treatment technique based on activated sludge process is the most widely applied biological wastewater treatment technique. With the development of modern molecular biological technology, it enhances our knowledge about the complexity and the biodiversity of activated sludge microbial communities. Numerous key microorganisms in activated sludge which were not detected by traditional cultivation methods, were disclosed by mod-ern biological techniques. These modern molecular biological techniques are represented by FISH, DGGE/TGGE, T-RFLP, RAPD, AFLP, PCR-SSCP and so on. It reveals the characteristics and development of the modern techniques, and it provides guidance for study of activated sludge microbial community diversity.
文章引用：孙连鹏, 崔语涵, 黄剑明, 叶挺进, 程毅. 分子生物技术在污泥微生物群落多样性研究中的应用—污泥微生物群落研究[J]. 生物过程, 2012, 2(1): 13-20. http://dx.doi.org/10.12677/bp.2012.21003

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