“绿岭”核桃种胚组培苗腋芽快繁体系的初步研究

Rapid Propagation of “Lv Ling” Walnut (Juglans Regia L.) through Embryo-Induced Seedlings

摘要:

以早实薄皮核桃“绿岭”成熟种胚诱导出的腋芽为外植体，MS为基本培养基，通过调节激素种类和浓度配比，初步研究了“绿岭”核桃种胚组培苗腋芽快繁体系。比较试验结果表明：最佳种胚萌发培养基为MS + 蔗糖30 g/L + 琼脂6 g/L；最佳腋芽诱导和增殖培养基为MS + 6-BA 2.0mg/L + NAA 0.2 mg/L + 蔗糖30 g/L + 琼脂6 g/L。生根采用两步生根法：先在DKW + IBA 8.0 mg/L + 蔗糖30 g/L + 琼脂6.0 g/L暗培养20 d，然后转入DKW + 蔗糖30 g/L + 琼脂6 g/L培养基中进行光照培养，生根率为50%。诱导生根的组培苗移栽到大田后成活率为33.3%。

Abstract: The mature embryo induced axillary buds were used as explants to investigate rapid propagation of precocious thin-shelled “Lv Ling” walnut in the MS basic medium with different concentrations of plant growth regulators. The results showed that MS + sucrose 30 g/L + agar6 g/L was the most feasible medium for embryo germination, and MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L + sucrose30 g/L + agar6 g/L was the best for induction and multiplication of the axillary buds in all tested treatments. The 50% of rooting rate was achieved by a two-stage rooting method, namely, the shoots were first cultivated in DKW + IBA 8.0 mg/L + sucrose30 g/L + agar6.0 g/L medium for 20 days under dark condition, then transferred to DKW+ sucrose30 g/L + agar6.0 g/L medium under light condition. After transplanting to the field, 33.3% of the seedlings survived.