大肠杆菌基因缺失菌株中碳中心代谢系统的通量平衡分析和通量变异分析

FBA and FVA Analysis of Central Carbon Metabolism in E. coli Deletion Mutants

摘要: 大肠杆菌(E. coli)因其遗传信息简单且容易培养而被广泛用作各种生物化学、生物技术研究等的模式微生物。本文首先通过COBRA工具包构建了大肠杆菌碳中心代谢系统的小规模网络，然后分别模拟敲除了其中的25种酶，并利用代谢通量平衡分析和代谢通量变异分析研究了基因敲除后的代谢网络。通过这些结果的综合分析，我们可以推断大肠杆菌碳中心代谢系统的不同的酶对整个体系的作用。

Abstract: E. coli has clear genetic background and is easy to be cultured, so it has been widely used in a variety of biochemistry, biotechnology research as the mode microorganisms. In this work, a small E. coli metabolic system has been built in the cobra workspace. 25 enzymes in the metabolic system were separately knocked out and the metabolic system was investigated by FBA (flux balance analysis) and FVA (flux variability analysis). By combing the outcome of the analysis, we could infer the role played by the various enzymes in the E. coli metabolic system.