分子生物学技术在土壤微生物多样性中的应用
Molecular Biology Technology and Its Application in Soil Microbial Diversity
DOI: 10.12677/BP.2013.33004, PDF, HTML, XML,  被引量 下载: 4,532  浏览: 17,957  国家自然科学基金支持
作者: 曹万举：吉林省白河林业局；隋 心：中国科学院沈阳应用生态研究所；中国科学院大学；韩士杰：中国科学院沈阳应用生态研究所；王 云, 李金功, 王洁：吉林省长白山自然保护管理中心
关键词: 微生物多样性；研究方法；分子生物学；生态系统；Microbial Diversity; Research Method; Molecular Biology; Ecosystems
摘要: 微生物在生态系统中占据着很重要的作用，其结构和功能的多样性及变化在一定程度上反映了生态系统的基本状态。传统的微生物培养和鉴定方法得到的微生物信息很片面，不足以代表微生物在生态系统中的真实情况。近几十年发展起来的分子生物技术突破了传统方法的限制，极大地促进了微生物学和生态学的发展。本文介绍了现在常用的几种分子生物学方法在微生物多样性研究中的应用现状。
Abstract: Microorganism plays a key role in ecosystem, the structure and function of microbial diversity, to a certain extent, reflects the status of ecosystem. It is unilateral to study microorganism with traditional cultural method, which could not reflect the real situation of microorganism in ecosystem. Recently, the molecular biology technology breaks the limitation of traditional cultural method and tremendously promotes the development of microbiology and ecology. So, this paper introduces several molecular biology methods which they’re applied in microbial diversity research.

文章引用：曹万举, 隋心, 韩士杰, 王云, 李金功, 王洁. 分子生物学技术在土壤微生物多样性中的应用[J]. 生物过程, 2013, 3(3): 23-28. http://dx.doi.org/10.12677/BP.2013.33004

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