铁化羧甲基壳聚糖的抗氧化作用研究

Synthesis and Antioxidant Activity of Carboxymethyl Chitosan-Fe

摘要: 目前的补铁剂正在向多糖铁方向发展，本文在前期成功合成了铁化羧甲基壳聚糖的基础上，采用邻苯三酚自氧化法、DPPH法测定铁化羧甲基壳聚糖的抗氧化能力。结果表明，在实验设置的浓度范围0.125 mg/mL~2 mg/mL内，铁化羧甲基壳聚糖的抗氧化能力随着浓度的增加而增加，且铁化羧甲基壳聚糖的抗氧化能力高于羧甲基壳聚糖。2.0 mg/mL的铁化羧甲基壳聚糖对超氧阴离子自由基的清除率为62.68%，对DPPH自由基的清除率为56.12%。结果提示，铁化羧甲基壳聚糖有望开发成为一种具有多重功能的生物多糖型补铁剂。

Abstract: The antioxidant activities in vitro of carboxymethyl chitosan-Fe which was synthesized before in our lab were investigated by pyrogallol autoxidation and DPPH method. The results showed that in the setting concentration range: 0.125 mg/mL - 2 mg/mL, the antioxidant activities increased with the elevation of concentration. The antioxidant activities of carboxymethyl chitosan-Fe were better than those of carboxymethyl chitosan. The clearance rates of superoxide anion free radical and DPPH of 2.0mg/mL carboxymethyl chitosan-Fe were 62.68% and 56.12%. The results proved the successful synthesis of carboxymethyl chitosan-Fe and it may become a multi-function organic ferruginous polysaccharide type.