MD  >> Vol. 4 No. 4 (December 2014)

    Study of the Change of Serous Inflammatory Factor IL-8 and Clinical Significance after Burn Injury

  • 全文下载: PDF(378KB) HTML    PP.44-48   DOI: 10.12677/MD.2014.44008  
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烧伤血清炎症因子白介素-8病情评估Burn Inflammatory Factor IL-8 Evaluating Burn Condition


目的:检测烧伤后患者体内炎症因子IL-8值在不同时间点的变化情况,研究其在烧伤后的变化规律,探讨烧伤后检测IL-8值的临床意义。方法:选择烧伤患者86例,按照烧伤总体表面积不同分为三组,分别收集烧伤后1、3、7、14、21天患者的血清,利用酶联免疫吸附试验法(ELISA)检测血清中IL-8的值,与45例健康献血人员血清IL-8值对照比较。结果:(1) 与对照组相比,I组、II组患者血清炎症因子IL-8值在烧伤后1、3、7天时均显著增高(P < 0.01),I组烧伤患者血清IL-8值在14、21天下降,而II组患者血清IL-8值在14、21天仍维持高位,与对照组比较具有差异(P < 0.01)。(2) I组烧伤患者血清IL-8值在烧伤后3天达峰值,其后均不同程度下降,II组烧伤患者血清IL-8值在伤后14天又有一个峰值。(3) 随着烧伤患者病情的变化,烧伤患者血清IL-8值发生不同的变化趋势。结论:烧伤后患者体内的炎症因子IL-8值明显升高。炎症因子IL-8值在烧伤后不同时间点的变化,同体表烧伤面积、病情变化及烧伤愈合程度等多种情况有关,在病情分级的基础上早期联合监测烧伤患者血清炎症因子IL-8值的变化,对判断烧伤病情的严重程度、协助指导烧伤的治疗、评估烧伤病情的转归和判断预后具有一定的帮助价值。

Objective: To detect the change of serous inflammatory factor IL-8 at different time points after burn injury, study its change rule and explore the clinical significance. Methods: We choose 86 cases of burn patients, and according to the total burn surface area, they are divided into three groups, respectively collect the patient’s serum of burns after 1, 3, 7, 14, 21 days, using the method of enzyme linked immunosorbent assay (ELISA) to detect the levels of serum IL-8, and compare with the 45 cases of healthy blood serum. Results: (1) Compared with the control group, the level of IL-8 in I and II group was significantly increased (P < 0.01) after burn 1, 3, 7 days. The level of IL-8 in I group was declined gradually after burn 14, 21 days, while II group patients IL-8 values still remain high at 14, 21 days, there was difference with the control group (P < 0.01). (3) With the change of burn patients, the level of IL-8 has different trend. Conclusions: The value of IL-8 in burn patients is obviously increased. On the basis of the disease classification, monitoring the value of IL-8 change can help to judge the severity of the burn condition, to guide the treatment of burns and to evaluate the burn the outcome.

梁建国, 梁发汤, 刘立民, 王祖峰. 烧伤后血清炎症因子白介素-8值的变化与临床意义[J]. 医学诊断, 2014, 4(4): 44-48.


[1] Allen, T.C. and Kurdowska, A. (2014) Interleukin 8 and acute lung injury. Archives of Pathology & Laboratory Medicine, 138, 266-269.
[2] Palena, C., Hamilton, D.H. and Fernando, R.I. (2012) Influence of IL-8 on the epitheli-al-mesenchymal transition and the tumor microenvironment. Future Oncology, 8, 713-722.
[3] 潘宇红, 黄璇, 丁羚涛, 等 (2013) 严重烧伤患者促炎和抗炎细胞因子的变化及其意义. 国际检验医学杂志, 34, 29-31.
[4] Mace, J.E., Park, M.S., Mora, A.G., et al. (2012) Differential expression of the immunoinflammatory response in trauma patients: Burn vs. non-burn. Burns, 38, 599-606.
[5] Noronha, S.M., Noronha, S.A., Klepp, A.G., et al. (2014) Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients. Acta Cirurgica Brasileira, 29, 62-68.
[6] 刘梅, 贺飞燕 (2012) 白细胞介素-8基因多态性与疾病关系的研究进展. 现代生物医学进展, 9, 1769-1771.
[7] Kanai, T., Kamada, N. and Hisamatsu, T. (2013) Clinical strategies for the blockade of IL-18 in inflammatory bowel diseases. Current Drug Targets, 14, 1392-1399.
[8] 姚瑶, 王琳琳 (2009) 白细胞介素-8研究进展. 实用儿科杂志, 10, 789-791.
[9] Lotti, F. and Maggi, M. (2013) Interleukin-8 and the male genital tract. Journal of Reproductive Immunology, 100, 54- 65.
[10] Pichert, A., Schlorke, D., Franz, S., et al. (2012) Functional aspects of the interaction between interleukin-8 and sulfated glycosaminoglycans. Biomatter, 2, 142-148.
[11] Kotyza, J. (2012) Interleukin-8 (CXCL8) in tumor associated non-vascular extracellular fluids: Its diagnostic and prognostic values. The International Journal of Biological Markers, 27, 169-178.
[12] Mendonça Machado, N., Gragnani, A. and Masako Ferreira, L. (2011) Burns, metabolism and nutritional requirements. Nutrición Hospitalaria, 26, 692-700.
[13] Rojas, Y., Finnerty, C.C., Radhakrishnan, R.S., et al. (2012) Burns: An update on current pharmacotherapy. Expert Opinion on Pharmacotherapy, 13, 2485-2494.
[14] Xiu, F. and Jeschke, M.G. (2013) Perturbed mononuclear phagocyte system in severely burned and septic patients. Shock, 40, 81-88.