化学工程与技术  >> Vol. 5 No. 2 (March 2015)

分散液相微萃取技术在氯胺酮检验中的应用
The Dispersive Liquid-Liquid Microextraction Technology in the Application of Ketamine Inspection

DOI: 10.12677/HJCET.2015.52006, PDF, HTML, XML, 下载: 2,032  浏览: 7,298  科研立项经费支持

作者: 彭 鹏, 周朝阳, 桑骏翔, 周彬斌:江苏警官学院,江苏 南京

关键词: 分散液相微萃取气相色谱氯胺酮Dispersive Liquid-Liquid Microextraction Gas Chromatography Ketamine

摘要: 本文利用分散液相微萃取技术(DLLME)与气相色谱法(GC)相结合,建立检验氯胺酮的定性定量分析的新方法。将含有40.0 µL二氯甲烷(萃取剂)的0.5 mL异丙醇(分散剂)作为萃取体系,快速注入到1.0 mL的水溶液中。分散均匀后,以6000 r/min离心5 min后得到沉于离心管底部尖端的沉淀相(15 ± 0.5 µL),取底部沉淀相1.0 µL进行气相色谱分析。方法线性范围1.0~900.0 µg/L (r = 0.9996),检出限0.085 µg/L,相对标准偏差3.5% (n = 3),加标平均回收率94.2%。
Abstract: By combining DLLME and GC phase, a new method for qualitative and quantitative analysis of the inspection of ketamine is presented. A mixture of 40.0 µL dichloromethane and 0.5 mL isopropyl alcohol is the extraction system. First, we quickly add the mixture to 1.0 mL pure water. Then, we centrifuge for 5 min at 6000 r/min speed in a microcentrifuge. There are 15 ± 0.5 µL precipitated phase at the bottom of the centrifuge tube. Last, we analyze 1 mL precipitated phase by gas chro-matography. The linear range of the method is 1.0 - 900.0 µg/L (r = 0.9996). The detection limit of ketamine is 0.085 µg/L. The relative standard deviation of the determination is 3.5% (n = 3). The average recovery rate is 94.2% to add ketamine into the samples.

文章引用: 彭鹏, 周朝阳, 桑骏翔, 周彬斌. 分散液相微萃取技术在氯胺酮检验中的应用[J]. 化学工程与技术, 2015, 5(2): 33-39. http://dx.doi.org/10.12677/HJCET.2015.52006

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