基于葡萄糖氧化酶/介孔碳复合材料的葡萄糖生物传感器
Glucose Biosensor Based on Glucose Oxidase/Ordered Mesoporous Carbon Composite Material
DOI: 10.12677/JSTA.2015.33009, PDF, HTML, XML, 下载: 2,625  浏览: 10,760  国家科技经费支持
作者: 唐悦, 魏倾鹤, 梁羽佳, 吉艳*, 齐斌*：东北师范大学化学学院，吉林 长春
关键词: 有序介孔碳；葡萄糖氧化酶；葡萄糖；生物传感器；Ordered Mesoporous Carbon； GOD； Glocuse； Biosensors
摘要: 我们采用浸渍法将GOD吸附在有序介孔碳修饰的电极表面(GOD/OMC/GCE)，然后又在其表面覆盖了一层二茂铁丙酮溶液，最终成功构建了一种新的电流型葡萄糖生物传感器。结果表明，该修饰电极Fc/GOD/OMC/GCE对葡萄糖有很好的电催化活性，对葡萄糖的检测线性范围为5.00 μM~10.00 mM；检出限为1.03 ± 0.15 μM (信噪比为3)。同时对该修饰电极的选择性与稳定性也做了讨论。
Abstract: Glucose oxidase (GOD) was adsorbed on the surface of OMC modified glassy carbon electrode (GOD/OMC/GCE). Then ferrocene was assembled on the GOD/OMC/GCE platform to construct an amperometric glucose biosensor. The results showed that the Fc/GOD/OMC nanocomposite exhibits a remarkably improved catalytic activity towards the oxidation of glucose. The biosensor exhibited a wide linear response up to 10 mM with a low detection limit of 1.03 ± 0.15 μM (S/N = 3). The selectivity and stability of the biosensor were also investigated.
文章引用：唐悦, 魏倾鹤, 梁羽佳, 吉艳, 齐斌. 基于葡萄糖氧化酶/介孔碳复合材料的葡萄糖生物传感器[J]. 传感器技术与应用, 2015, 3(3): 69-78. http://dx.doi.org/10.12677/JSTA.2015.33009

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