传感器技术与应用  >> Vol. 4 No. 1 (January 2016)

基于微流控CL芯片的厚朴药材提取物的抗氧化活性分析
Magnolia officinalis Extract Antioxidant Activity Analysis Based on Microfluidic CL Chip

DOI: 10.12677/JSTA.2016.41003, PDF, HTML, XML, 下载: 1,617  浏览: 3,890  科研立项经费支持

作者: 张涛, 张潇丹, 王蓉, 张贝, 徐溢*:重庆大学化学化工学院,重庆;新型微纳器件与系统技术重点 学科实验室,重庆;微纳系统及新材料技术国际联合研发中心 ,重庆;吕君江:重庆大学化学化工学院,重庆

关键词: 厚朴抗氧化性化学发光微流控芯片Magnolia Antioxidant Activity Chemiluminescence Microfluidic Chip

摘要: 设计并制作了一种集成有四个重复结构单元的微流控化学发光(chemiluminescence, CL)芯片,以实现中药材厚朴提取物的分子水平的抗氧化活性分析。芯片结构单元包含蛇形通道、高效环形混合通道和椭圆形检测区。微流控CL芯片系统一次可完成单一样本多浓度的检测。采用luminol-H2O2-Cu2+化学发光体系对厚朴酚、和厚朴酚标品进行CL测试以验证该芯片可靠性,并得出最佳测试条件为:Luninol浓度为0.50 mmol∙L−1,H2O2浓度为1.00 mmol∙L−1,Cu2+浓度为0.50 mmol∙L−1,流速10.0 µL∙min−1。进而对厚朴药材五种醇提物进行CL测试,结果表明,五种提取物对H2O2均有一定的清除作用,且1.00 g∙ml−1的五种提取物的当量抗氧化活性强弱顺序为:80%乙醇提取物 > 60%乙醇提取物 > 40%乙醇提取物 > 20%乙醇提取物 > 水提取物。
Abstract: An integrated microfluidic chip with four repeating structural units was designed and fabricated to achieve molecular level of Magnolia medicines extracts antioxidant activity analysis. Chip structure unit includes a serpentine channel, efficient mixing channel and an oval ring detection zone. The Microfluidic chemiluminescence chip system could successfully complete synchronous detection of single sample with different concentration. Using luminol-H2O2-Cu2+chemiluminescence system for Magnolol, Honokiol CL-standard products were tested to verify the reliability of the chip, and optimum test conditions are: luminol concentration is 0.50 mmol∙L−1, the H2O2 concentration is 1.00 mmol∙L−1, Cu2+ concentration is 0.50 mmol∙L−1 and the flow rate is 10.0 μL∙min−1. Furthermore Magnolia officinalis extracts from five alcohol antioxidant activity test results showed that the five extracts antioxidant activity, which was extracted under different condition from 1.00 g∙mL−1 Magnolia officinalis, has a certain effect to remove H2O2, and the order of antioxidant activity strength was: 80% ethanol extract > 60% ethanol extract > 40% ethanol extract> 20% ethanol extract> aqueous extract.

文章引用: 张涛, 张潇丹, 王蓉, 张贝, 吕君江, 徐溢. 基于微流控CL芯片的厚朴药材提取物的抗氧化活性分析[J]. 传感器技术与应用, 2016, 4(1): 15-24. http://dx.doi.org/10.12677/JSTA.2016.41003

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