NAT  >> Vol. 6 No. 1 (February 2016)

    量子点作为荧光探针在生物医学领域的研究进展
    Advances of Quantum Dots as Fluorescent Probes in Biological and Medical Fields

  • 全文下载: PDF(397KB) HTML   XML   PP.9-13   DOI: 10.12677/NAT.2016.61002  
  • 下载量: 1,525  浏览量: 4,181  

作者:  

宋国龙,孔祥东:浙江理工大学生命科学学院,生物材料与海洋生物资源研究所,浙江 杭州

关键词:
量子点生物探针活体成像Quantum Dots Biological Probes In Vivo Imaging

摘要:

量子点是一种三维纳米晶粒,具有许多优异的光学性能,如激发波长范围宽、发射波长范围窄且对称、量子产率高、荧光寿命长、光学性能稳定等优点。量子点可以作为荧光探针来标记生物体系的不同成分,如组织、细胞、生物大分子以及动物活体成像,本文就量子点在生物医学领域作为荧光标记物的研究进展加以综述。

Quantum dots (QDs), three-dimensional (3-D) nanocrystals, possess a great deal of unique optical performances, such as wide excitation wavelength, narrow and symmetric emission wavelength, high quantum yield, long fluorescence lifespan, stable optical property. QDs can be used as fluorescent probes to label different components in biosystem, which contains tissues, cells, molecules and living animals imaging. A review on the advances of QDs as fluorescent probes in Biological and Medical fields is given in the paper.

文章引用:
宋国龙, 孔祥东. 量子点作为荧光探针在生物医学领域的研究进展[J]. 纳米技术, 2016, 6(1): 9-13. http://dx.doi.org/10.12677/NAT.2016.61002

参考文献

[1] Bruchez, M., Moronne, M., Gin, P., Weiss, S. and Alivisatos, A.P. (1998) Semiconductor Nanocrystals as Fluorescent Biological Labels. Science, 281, 2013-2016.
http://dx.doi.org/10.1126/science.281.5385.2013
[2] Chan, W.C. and Nie, S. (1998) Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection. Science, 281, 2016-2018.
http://dx.doi.org/10.1126/science.281.5385.2016
[3] Lingerfelt, B.M., Mattoussi, H., Goldman, E.R., Mauro, J.M. and Anderson, G.P. (2003) Preparation of Quantum Dot- Biotin Conjugates and Their Use in Immunochromatography Assays. Analytical Chemistry, 75, 4043-4049.
http://dx.doi.org/10.1021/ac034139e
[4] Chang, E., Miller, J.S., Sun, J., Yu, W.W., Colvin, V.L., Drezek, R., et al. (2005) Protease-Activated Quantum Dot Probes. Biochemical and Biophysical Research Communications, 334, 1317-1321.
http://dx.doi.org/10.1016/j.bbrc.2005.07.028
[5] Parak, W.J., Gerion, D., Zanchet, D., Woerz, A.S., Pellegrino, T., Micheel, C., et al. (2002) Conjugation of DNA to Silanized Colloidal Semiconductor Nanocrystalline Quantum Dots. Chemistry of Materials, 14, 2113-2119.
http://dx.doi.org/10.1021/cm0107878
[6] Ebenstein, Y., Gassman, N., Kim, S., Antelman, J., Kim, Y., Ho, S., et al. (2009) Lighting up Individual DNA Binding Proteins with Quantum Dots. Nano Letters, 9, 1598-1603.
http://dx.doi.org/10.1021/nl803820b
[7] Song, Y., Li, Y., Liu, Z., Liu, L., Wang, X., Su, X., et al. (2014) A Novel Ultrasensitive Carboxymethyl Chitosan- Quantum Dot-Based Fluorescence “Turn on-off” Nanosensor for Lysozyme Detection. Biosensors & Bioelectronics, 61, 9-13.
http://dx.doi.org/10.1016/j.bios.2014.04.036
[8] Zhang, H., Sun, P., Liu, C., Gao, H., Xu, L., Fang, J., et al. (2011) L-Cysteine Capped CdTe-CdS Core-Shell Quantum Dots: Preparation, Characterization and Immuno-Labeling of HeLa Cells. Luminescence: The Journal of Biological and Chemical Luminescence, 26, 86-92.
http://dx.doi.org/10.1002/bio.1188
[9] Chen, F. and Gerion, D. (2004) Fluorescent CdSe/ZnS Nanocrystal-Peptide Conjugates for Long-Term, Nontoxic Imaging and Nuclear Targeting in Living Cells. Nano Letters, 4, 1827-1832.
http://dx.doi.org/10.1021/nl049170q
[10] Gao, J. and Xu, B. (2009) Applications of Nanomaterials inside Cells. Nano Today, 4, 37-51.
http://dx.doi.org/10.1016/j.nantod.2008.10.009
[11] Dong, W., Ge, X., Wang, M. and Xu, S. (2010) Labeling of BSA and Imaging of Mouse T-Lymphocyte as Well as Mouse Spleen Tissue by l-Glutathione Capped CdTe Quantum Dots. Luminescence: The Journal of Biological and Chemical Luminescence, 25, 55-60.
[12] Lim, Y.T., Cho, M.Y., Noh, Y.-W., Chung, J.W. and Chung, B.H. (2009) Near-Infrared Emitting Fluorescent Nanocrystals-Labeled Natural Killer Cells as a Platform Technology for the Optical Imaging of Immunotherapeutic Cells- Based Cancer Therapy. Nanotechnology, 20, 475102.
http://dx.doi.org/10.1088/0957-4484/20/47/475102
[13] Gao, X., Cui, Y., Levenson, R.M., Chung, L.W. and Nie, S. (2004) In Vivo Cancer Targeting and Imaging with Semiconductor Quantum Dots. Nature Biotechnology, 22, 969-976.
http://dx.doi.org/10.1038/nbt994
[14] Nida, D.L., Rahman, M.S., Carlson, K.D., Richards-Kortum, R. and Follen, M. (2005) Fluorescent Nanocrystals for Use in Early Cervical Cancer Detection. Gynecologic Oncology, 99, S89-S94.
http://dx.doi.org/10.1016/j.ygyno.2005.07.050
[15] Santra, S., Yang, H., Stanley, J.T., Holloway, P.H., Moudgil, B.M., Walter, G., et al. (2005) Rapid and Effective Labeling of Brain Tissue Using TAT-Conjugated CdS:Mn/ZnS Quantum Dots. Chemical Communications. 2005, 3144- 3146.
http://dx.doi.org/10.1039/b503234b
[16] Ravindran, S., Kim, S., Martin, R., Lord, E.M. and Ozkan, C.S. (2005) Quantum Dots as Bio-Labels for the Localization of a Small Plant Adhesion Protein. Nanotechnology, 16, 1.
http://dx.doi.org/10.1088/0957-4484/16/1/001