OJNS  >> Vol. 4 No. 2 (May 2016)

    Study on DNA Extraction Methods for Minute Touch Biological Samples

  • 全文下载: PDF(405KB) HTML   XML   PP.156-162   DOI: 10.12677/OJNS.2016.42019  
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皇甫一润,杨晓蒋,曹晨曦,黄 莉:江苏警官学院,江苏 南京;
孟 昕:南京市公安局玄武分局刑大,江苏 南京

微量接触性生物检材脱落细胞转移DNA提取Microscale Contact (Touch) Biomaterial (Biological Samples) The Transfer of Cell Detach DNA Extractions



“TES solution two-step extraction method”, “NaCl solution two-step extraction method” and “Double-sided adhesive paste method” are three methods we used to detach and transfer cells. Our goal is to examine and compare the test results among three detaching methods above so that we are able to provide references for optimizing the extraction method in the future. Method: After applying three cell extraction methods above to 168 sweat samples located on glasses, aluminum, plastic and wood, we can use Chelex method or silicon bead test to extract the DNA from these samples. Furthermore, after applying PCR amplification, routine STR testing, we are able to validate the test result using fingerprints. Result: “NaCl solution two-step extraction method” combined with silicon bead test is the best biomaterial extraction method and it is capable for public security practice.

皇甫一润, 孟昕, 杨晓蒋, 曹晨曦, 黄莉. 微量接触性生物检材提取方法研究[J]. 自然科学, 2016, 4(2): 156-162. http://dx.doi.org/10.12677/OJNS.2016.42019