摘要:
胚胎细胞具有活跃的分裂增殖能力和多向分化潜能，是建立细胞系的最佳组织来源。但是由于微生物和原生动物污染以及体外培养条件下细胞不分裂等原因，海洋无脊椎动物的细胞培养研究目前仍停留在原代细胞培养和有限传代培养的水平上，尚无永生性细胞系的成功报道。本文综述了海洋无脊椎动物中5个门类包括多孔动物门、腔肠动物门、节肢动物门、软体动物门和棘皮动物门的胚胎细胞培养工作的研究进展，并对海洋无脊椎动物胚胎细胞培养的前景进行了展望。
Abstract:
Embryo cells with high potential in active proliferation and pluripotency are optimal cell sources for the establishment of continuous cell lines. However, due to the frequent occurrence of con-tamination by microorganisms and protozoans as well as the mitosis-arrestment of primary cul- ture cells, only primary cell cultures and limited subculture are successful in the cell culture of invertebrates, and no immortalized cell line has been established from invertebrates. This paper has reviewed the advances in the embryonic cell culture of marine invertebrates including Porifera, Coelenterata, Arthropoda, Mollusca and Echinodermata, and predicted its future outlook.