摘要:
目的：探讨母鼠妊娠后经历慢性轻度不可预计应激对其子代学习记忆和焦虑行为的影响。方法：应激组母鼠在怀孕时进行慢性应激干预，每日随机选取两种刺激(如湿笼、禁水食、束缚刺激等)；而对照组母鼠不进行任何处理。子代大鼠于青春前期(28 d)和青春后期(49 d)进行Morris水迷宫试验；并在青春期后期(49 d)进行旷场实验。结果：对照组子鼠相比，应激组子鼠体重增长明显减缓(F = 13.098, *p < 0.05)，在青春期中期(42 d~49 d)有较大的差异(42 d: F = 1.000, **P < 0.001；47 d: F = 0.173, **P < 0.001)；青春前期(28 d)和青春后期(49 d)应激组子鼠在寻找平台的潜伏期较对照组没有明显差异，在青春期前期，对照组与应激组子鼠在时间上并没有显著差异(F = 0.246, P = 0.695)，但是在路程上具差异显著具有统计学意义(F = 4.016, *P = 0.002)；而在青春期后期，对照组与应激组子鼠在时间和路程上都差异不显著，无统计学意义；并且应激组子鼠的水平活动能力与垂直活动能力明显少于对照组子鼠(穿格数：F = 0.1543，**P < 0.001；直立次数：F = 1.764，P = 0.002；梳理次数：F = 1.287，**P < 0.001)。结论：产前应激会损伤子代大鼠的记忆认知的发展并导致幼儿期及青春期学习记忆能力的减退，易引发焦虑情绪和行为。
Abstract:
Objective: To determine the effect of prenatal chronic unpredictable mild stress (CUS) during the pregnancy on the behavior parameters of learning and memory and anxiety of the rat offspring. Methods: The prenatal CUS model was adopted. During pregnancy to fertility, the mothers of CUS group underwent two stimuli daily randomly (such as wet, 45˚ inclined cage, cage ban water feed, bound to stimulate, etc.); While the mothers of control group didn’t deal with any processing. The offspring rats from each group were examined in Morris Water Maze at preadolescence (28 d) and postpubescence (49 d), and examined the spontaneous behavior and anxiety behavior by the open-field test at postpubescence (49 d). Results: CUS influences on growth of rat body weight (F = 13.098, *P < 0.05), in the middle of the puberty (42 d - 49 d), have bigger difference (42 d: F = 1.000, **P < 0.001; 47 d: F = 0.173, **P < 0.001), and there was no significant difference in the search for the platform of the incubation period between the offspring rats of stress group and offspring rats of control group offspring at preadolescence (28 d) and postpubescence (49 d) (F = 0.246, P = 0.695), while there was also a difference in the search strategy of straight and random patterns in postpubescence (49 d) offspring rats (F = 4.016, *P < 0.05). The horizontal and vertical mobility of the offspring rats of stress group was less than the offspring rats of control group, the difference is statistically significant (Crossing: F = 0.1543, **P < 0.001; Upright: F = 1.764, P = 0.002; Comb: F = 1.287, **P < 0.001). Conclusion: Prenatal stress will affect the development of the offspring rats memory cognitive, damage the ability of learning and memory of early childhood and adolescence, and easy to cause anxiety.