小鼠皮肤烫伤模型的建立

Establishment of Skin Scald Model in Mice

摘要: 建立用于皮肤烫伤修复药物研发的动物模型。将20只实验组BALB/c小鼠麻醉后，分别用沸水(接触时间为10 s)在小鼠背部烫出直径1 cm的圆形烫伤区，烫伤后1、7、14和21 d处死小鼠，取其损伤皮肤进行组织学的观察。组织学观察发现烫伤1 d的小鼠皮肤全层组织细胞发生水肿，符合Ⅱ度烫伤特点，7 d时小鼠皮肤组织细胞发生坏死并出现炎性反应，14 d和21 d时烫伤皮肤处开始出现增生肉芽组织。所有数据均表明本研究采用简单的方法成功制作出较为稳定的皮肤烫伤模型，从而为后续进行皮肤烫伤药物的开发奠定了一定的理论基础。

Abstract: To establish an animal model in mice for the drug development with skin scald. After the anesthe-tization BABL/c mice were scalded in a 1 cm-diameter circle area on the dorsum by boiling water at contact time 10 s. The mice were sacrificed at 1, 7, 14 and 21 days after scald. The skin samples were collected and analyzed by histopathological examinations. Histological observation showed a II degree injury involving full-thickness skin in the 1 d mice group. Tissue necrosis and inflammatory reaction were observed in the 7 d mice group. Granulation tissue hyperplasia began to appear in the 14 d and 21 d mice group. All the results showed that a mice skin scald model was successfully established which is stable and can be used on the drug development for the damaged skin in future research.