不同仪器测定化妆品中氯霉素的含量

Different Instrument for Determining the Content of Chloramphenicol in Cosmetics

摘要: 目的：采用三种不同液相色谱仪，对祛痘除螨类化妆品中氯霉素含量进行测定。方法：在相同的实验条件下，以氯霉素为标准物质，C₁₈柱(250 mm × 4.6 mm, 5 µm)为分离柱，(甲醇 + 乙腈 = 1:2):0.01 mol/L草酸(pH = 2.0) = 40：60为流动相洗脱，二极管阵列检测器，检测波长268 nm，流速：0.8 mL/min。结果：L-3000、LC-20AT、L-6000测定的线性相关系数r分别为0.9976、0.9994、0.9998，精密度的保留时间与峰面积RSD%依次分别为1.8%和2.1%、0.85%和0.91%、0.50%和0.72%，稳定性RSD%分别为2.3%、0.37%、0.16%，重复性RSD%分别为2.7%、0.86%、0.57%，回收率和RSD%依次分别为96.2%、2.2%；97.4%、1.3%；99.3%、0.7%。结论：经比较，使用超高效液相色谱仪测定氯霉素灵敏度低、精密度高、重复性好。

Abstract: Objective: Using three different liquid chromatographs, to determine the chloramphenicol content in cosmetics for removing acne and acarus. Methods: Under the same experimental conditions, on the basis of chloramphenicol material, C₁₈ column (250 mm × 4.6 mm, 5 µm) was used as the sep-aration column, (methanol + acetonitrile = 1:2):0.01 mol/L oxalic acid (pH = 2.0) = 40:60 as mobile phase elution, and diode array detector was also used with detection wavelength of 268 nm, velocity of 0.8 mL/min. Results: The linear correlation coefficient r of L-3000, LC-20AT, L-6000 was 0.9976, 0.9994, 0.9998 respectively; RSD% of precision retention time and peak area were 1.8% and 2.1%, 1.8% and 0.91%, 0.50% and 0.72%, respectively; RSD% of stability was 2.3%, 0.37% and 2.3% respectively; RSD% of repetitive peak area was 2.7%, 0.86% and 2.7% respectively; the recovery rate and RSD% were 96.2% and 96.2%, 97.4% and 1.3%, 99.3% and 0.7% respectively. Conclusion: After comparing, using ultra performance liquid chromatography to determine chloramphenicol has advantages of low sensitivity, high precision and good repeatability.