摘要:
本文主要研究了不同浓度的菜籽油和亚麻籽油对粒毛盘菌YM406生物量和代谢产物的影响。向培养基中分别添加0.5%、1%、2%、3%和4%的菜籽油和亚麻籽油进行发酵10天，发现当菜籽油浓度为1%，亚麻籽油浓度为2%时，生物量与未添加油脂的平均生物量(4.29 g/L)差距不大，分别是4.26 g/L和4.27 g/L，而其他浓度都会不同程度地抑制生物量。但同一油脂不同浓度间生物量没有显著性差异。不同浓度的菜籽油和亚麻籽油对胞外多糖(LEP)产量均有明显的抑制作用。选择添加1%菜籽油和添加2%亚麻籽油的发酵液，采用液相色谱-质谱联用技术(LC-MS)，分别检测了经氯仿、乙酸乙酯萃取及未萃取的发酵液中代谢产物，检测结果显示添加1%菜籽油发酵液共检测出27种物质，添加2%亚麻籽油发酵液共检测出20种物质，添加这两种油脂的发酵液中检测出的物质只有4种相同，这说明不同类型的油脂对代谢产物类型有显著影响。添加1%菜籽油发酵液经两种萃取剂萃取后只有3种相同物质，而添加2%亚麻籽油后只有4种，说明不同萃取剂萃取后发酵液的检测结果差异明显。本研究结果表明，不同类型的油脂对粒毛盘菌YM406生物量和多糖产量具有抑制作用，并且不同浓度的油脂抑制作用不同；不同类型的油脂会影响粒毛盘菌YM406代谢产物种类。
Abstract:
The research mainly investigated the effects of different concentrations of rapeseed oil and linseed oil on biomass and metabolites of Lachnum sp. Different concentrations of 0.5%, 1%, 2%, 3% and 4% rapeseed oil or linseed oil were added into culture medium respectively and fermented for 10 days, it was found that the biomass of 1% rapeseed oil and 2% linseed oil were 4.26 g/L and 4.27 g/L, which were almost equal to that absence of lipids (4.29 g/L), while the biomass with rapeseed oil and linseed oil of other concentrations was inhibited at different degrees. However, there was no significant difference in the biomass of the same oil among different concentrations. Rapeseed oil and linseed oil in different concentrations could inhibit exopolysaccharides (LEP) production significantly. The fermentation broth with 1% rapeseed oil and 2% linseed oil was selected, then metabolites in chloroform and ethyl acetate extraction components of fermentation broth and the original one were analyzed by Liquid Chromatograph Mass Spectrometer (LC-MS), the results showed that 27 kinds of substances were detected in the fermentation broth with 1% rapeseed oil and 20 kinds of substances were detected in the one with 2% linseed oil, only 4 same substances were detected in the fermentation broth added with these two kinds of lipids, which suggested that different kinds of lipids had significant influences on metabolites. 3 same substances were detected in the fermentation broth with 1% rapeseed oil after extracted with two extraction solvents, while 4 same substances were detected in the fermentation broth (with 2% flax seed oil) extractions, which illustrated that the test results of different broth extractions were obviously different. The results of the research indicate that different kinds of lipids have inhibiting effects on biomass and LEP production of Lachnum and the inhibition effects of different concentrations of oil are different; different kinds of lipids can affect the types of metabolites of Lachnum.