海洋科学前沿  >> Vol. 3 No. 3 (September 2016)

磷酸盐对盐生杜氏藻生长和细胞色素积累的影响
Effects of Phosphate on the Growth and Cell Pigment Accumulation of Dunaliella salina

DOI: 10.12677/AMS.2016.33014, PDF, HTML, XML, 下载: 1,305  浏览: 3,108  国家科技经费支持

作者: 刘笑天, 王培磊:临沂大学生命科学学院,山东 临沂

关键词: KH2PO4/sub>盐生杜氏藻生长β-胡萝卜素吸收规律KH2PO4/sub> Dunaliella salina Growth β-Carotene Absorpted Patterns

摘要: 研究了不同浓度(0~0.30 mmol/L)的KH2PO4对盐生杜氏藻生长和细胞色素积累的影响,结果表明,添加0.10 mmol/L KH2PO4,细胞生长最快,最高密度为153 × 104 cell/mL,无磷对照组密度最低,仅为36 × 104 cell/mL;KH2PO4浓度为0.20 mmol/L时,β-胡萝卜素积累量最高,为12.8 mg/L,其次是0.15 mmol/L,对照组很低,仅为6.28 mg/L,KH2PO4超过0.25 mmol/L对β-胡萝卜素积累有抑制作用;适当添加KH2PO4对D. salina叶绿素a合成有促进作用;在培养的前6天,pH值明显上升,随后4~5天又下降;添加0.1 mmol/L KH2PO4细胞蛋白质含量最高,为37.34%,对照组仅为24.86%;0.10 mmol/L组的KH2PO4在6天内吸收完毕,KH2PO4吸收的时间与其最初的浓度呈正相关关系,建立了藻液中KH2PO4被盐生杜氏藻吸收的动力学方程。
Abstract: The effects of KH2PO4 with different levels on the growth and cell pigment accumulation of Dunaliel-la salina were investigated and the results indicated that the maximum growth of 153 × 104 cell/mL was detected in culture medium with 0.1 mmol/L KH2PO4 and the minimum of 36 × 104 cell/mL in the control group. The culture medium with 0.20 mmol/L KH2PO4 had the highest β-carotene con-tent of 12.8 mg/L, followed by 11.04 mg/L with 0.15 mmol/L KH2PO4, and the control group had lower β-carotene content of only 6.28 mg/L. Over 0.25 mmol/L KH2PO4 was found to have depress-ing function to cell growth. Right level KH2PO4 was helpful to chlorophyll a biosynthesis. During the first 6 days within culture period, pH values of the culture media rose up, and then declined in the following 4 - 5 days. The cells which were cultured in media with 0.10 mmol/L KH2PO4 had the high-est protein content of 37.34%, while the control group only had 24.86%; 0.10 mmol/L KH2PO4 was assimilated completely within 6 days, and the uptake time had right-relationship with KH2PO4 con-tents. Finally, the assimilated mechanic equation of KH2PO4 by D. salina was set up.

文章引用: 刘笑天, 王培磊. 磷酸盐对盐生杜氏藻生长和细胞色素积累的影响[J]. 海洋科学前沿, 2016, 3(3): 100-107. http://dx.doi.org/10.12677/AMS.2016.33014

参考文献

[1] 姜建国, 姚汝华. 氮磷比对盐藻生长及甘油和色素积累的影响[J]. 热带海洋, 1999, 18(1): 68-74.
[2] 郝建欣, 孙玉, 丛威. 不同因子胁迫盐藻积累β-胡萝卜素异构体的HPLC动态规律分析[J]. 海洋科学, 2003, 27(2): 41-49.
[3] Borowitzka, M.A., Borowitzka, L.J. and Kessly, D. (1990) Mass Culture of Dunaliella: from Laboratory to Pilot Plant. Journal of Applied Phycology, 2, 111-123.
[4] 陈晗华, 钱凯先. 氮磷对盐藻生长及其β-胡萝卜素积累的影响[J]. 浙江大学学报, 1997, 31(6): 732-735.
[5] Hejazi, M.A. and Wijffels, R.H. (1993) Cell Characteristics and Biochemical Composition of Dunaliella primolecta Butcher Conditioned at Different Concentration of Dissolved Nitrogen. Journal of Applied Phycology, 5, 447-453.
http://dx.doi.org/10.1007/BF02182737
[6] 李建宏, 翁永萍, 胡寒萍. 不同氮源对盐生杜氏藻生长和β-胡萝卜素积累的影响[J]. 南京师范大学学报, 1999, 22(3): 73-85.
[7] Thakur, A. and Kumar, H.D. (1999) Effects of pH, Light Quality and Various Carbon Sources on Glycerol Production by the Brackish Water Alga Dunalia salina. Cytobios, 97, 79-83.
[8] Laws, E.A. and Wong, D.C.L. (1978) Effect of Nitrate Concentration on Growth and Pigment Synthesis of Dunaliella salina Cultivated under Low Illumination and Preadapted to Different Salinities. Journal of Applied Phycology, 10, 406-411.
http://dx.doi.org/10.1111/j.1529-8817.1978.tb02460.x
[9] Giordano, M. and Bowes, G. (1997) A Salt-Induced Chloroplast Protein in Dunaliella salina (Chlorophyta). Plant Physiology, 115, 1049-1058.
[10] Hirsch, R., Carandang, J. and Treffny, B. (1992) Effect of Low Temperature on the Stereoisomer Composition of β-Carotene in the Halotolerant Alga Dunaliella bardawil (Chlorophyta). Journal of Experimental Botany, 43, 887-894.
http://dx.doi.org/10.1093/jxb/43.7.887
[11] Amotz, A.B. (1996) New Mode of Dunaliella Biotechnology: Two-Phase Growth for β-Carotene Production. Phycologia, 32, 272-284.