AAC  >> Vol. 6 No. 4 (November 2016)

    金果榄药材的UPLC指纹图谱研究
    Study on UPLC Fingerprint of Tinospora sagittata and T. capillipes

  • 全文下载: PDF(541KB) HTML   XML   PP.79-85   DOI: 10.12677/AAC.2016.64012  
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作者:  

李扬华,陈秋虹,梁兰娜,徐 慧,蒋艳芳:广西分析测试研究中心,广西 南宁

关键词:
金果榄UPLC指纹图谱质量控制 Tinospora sagittata and T. capillipes UPLC Fingerprints Quality Control

摘要:

目的:建立金果榄药材的UPLC指纹图谱,为金果榄药材的质量控制提供依据。方法:采用超高效液相色谱法。色谱条件:色谱柱:Acquity UPLC®BEN C18 (2.1 × 100 mm,1.7 µm);流动相A:乙腈;流动相B:0.3%磷酸溶液;流速:0.25 ml/min;检测波长:225 nm;柱温:25℃。结果:建立了10批金果榄药材的指纹图谱。金果榄药材有7个共有峰,多数峰可以达到较好分离,具有较高的相似度。结论:建立的超高效液相指纹图谱有较好的精密度、重复性和稳定性,可作为金果榄质量评价参考。

Objective: To establish UPLC fingerprint of Tinospora sagittata and T. capillipes which is expected to provide standards for the quality control and identification of the Chinese crude drug. Method: The UPLC method was used. Chromatography conditions were Acquity UPLC®BEN C18 (2.1 × 100 mm, 1.7 µm) column with gradient mobile phase of acetonitrile and 0.3% phosphate acid. UV detection wavelength was 225 nm, the flow rate was 0.25 ml/min and the column temperature was 25˚C. Result: UPLC fingerprints of 10 samples of Tinospora sagittata and T. capillipes were established. 7 mutual peaks were selected as the fingerprint peaks in the samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 10 samples had higher simi-larities. Conclusion: The established UPLC fingerprint has desirable accuracy, repeatability and sta-bility, which can be used as reference for the quality control of Tinospora sagittata and T. capillipes.

文章引用:
李扬华, 陈秋虹, 梁兰娜, 徐慧, 蒋艳芳. 金果榄药材的UPLC指纹图谱研究[J]. 分析化学进展, 2016, 6(4): 79-85. http://dx.doi.org/10.12677/AAC.2016.64012