HPLC法测定门冬氨酸鸟氨酸的含量

Determination of L-Ornithine-L-Asparate by HPLC

摘要: 目的：建立HPLC法测定门冬氨酸鸟氨酸的含量。方法：采用氨基键合硅胶色谱柱(250 mm × 4.6 mm，5 µm)，以0.05M磷酸二氢钾缓冲溶液–乙腈(40:60)为流动相；检测波长为210 nm；流速为1.0 ml/min；进样量为20 μL。结果：门冬氨酸鸟氨酸浓度在1.50~4.50 mg/mL范围内，即门冬氨酸浓度为0.75~2.26 mg/mL (r = 0.9998)，鸟氨酸浓度为0.75~2.24 mg/mL (r = 0.9998)，峰面积与浓度呈良好的线性关系。平均回收率为99.37%，RSD为0.34%。结论：该方法简便、快速、准确，适合于门冬氨酸鸟氨酸原料及制剂的含量测定。

Abstract: Objective: To establish a HPLC method for assay of L-Ornithine-L-Asparate. Methods: The HPLC method was conducted on Waters Spherisorb NH2 (250 mm × 4.6 mm, id 5 μm). The mobile phase was composed of acetonitrile-0.05 mol/L KH2PO4 (60:40) with the flow of 1.0 mL/min. The detection wave-length was 210 nm. Results: L-ornithine and L-aspartate showed good linear (r = 0.9998) in the range of 0.75 - 2.26 mg/mL and 0.75 - 2.24 mg/mL, respectively. The average recovery of L-Ornithine-L-Asparate was 99.37% (RSD = 0.34%). Conclusion: The method was simple, rapid and accurate. It is suitable for the assay of L-Ornithine-L-Asparate.