一株好氧反硝化菌的筛选鉴定及其脱氮特性研究
Identification and Denitrification Characteristics of an Isolated Aerobic Denitrifier
DOI: 10.12677/WPT.2017.51002, PDF, HTML, XML, 下载: 1,618  浏览: 3,347 
作者: 邵 可, 屈佳能, 邓寒梅, 阎光绪：中国石油大学(北京)，北京市油气污染防治重点实验室重质油国家重点实验室，北京
关键词: 好氧反硝化菌；分离与鉴定；反硝化能力；Aerobic Denitrifier； Isolation and Identification； Denitrifying Capacity
摘要: 本研究从巴陵石化己内酰胺污水中分离高效好氧反硝化菌，并对菌株的反硝化特性进行研究。通过BTB培养基初筛及菌株硝酸盐的降解实验，从样品中分离得到一株脱氮性能最好的好氧反硝化菌，结合其生理生化特性和16S rDNA测序结果，对菌株进行鉴定。研究不同碳源、C/N、温度及pH对其反硝化能力的影响。筛选得到高效好氧反硝化菌B-5，经鉴定属于假单胞菌属(Pseudomonas sp.)。降解特性研究表明，当碳源为琥珀酸钠或乙酸钠，C/N为15:1，温度为30℃~35℃，pH为6~7时，菌株B-5的降解性能最佳。
Abstract: This study aimed at the isolation of an aerobic denitrifier with high nitrogen removal ability from caprolactam sewage and its denitrifying characteristics. An aerobic denitrifier with the best deni-trifying performance was isolated from the sample by BTB medium preliminary screening and ni-trate degradation analysis. We used morphological and physiological characteristics as well as its 16S rDNA sequences to identify the strain. Effects of culture conditions, such as carbon sources, C/N ratio, temperature and initial pH, were evaluated regarding denitrifying capacity under aerobic condition. The highly effective strain B-5 was identified as Pseudomonas sp. Studies of degradation characteristics showed that the most efficient nitrate removal rate for B-5 occurred at C/N = 15 when sodium succinate or sodium acetate was supplemented as the carbon source. The optimum temperature for B-5 was 30˚C - 35˚C and optimum pH ranged from 6 to 7.
文章引用：邵可, 屈佳能, 邓寒梅, 阎光绪. 一株好氧反硝化菌的筛选鉴定及其脱氮特性研究[J]. 水污染及处理, 2017, 5(1): 6-14. http://dx.doi.org/10.12677/WPT.2017.51002

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