HPLC法同时测定香云肝泰片中鸟苷和腺苷的含量
Simultaneous Determination of Guanosine and Adenosine in Xiangyungantai by HPLC
DOI: 10.12677/PI.2017.61001, PDF, HTML, XML, 下载: 1,297  浏览: 3,005 
作者: 胡正明, 何银舟:江苏省淮安市淮安医院药剂科,江苏 淮安;柴 尧2, 嵇 晶, 刘 睿, 程建明*:南京中医药大学药学院,江苏 南京
关键词: 香云肝泰片高效液相色谱鸟苷腺苷Xiangyungantai HPLC Guanine Adenosine
摘要: 目的:分析香云肝泰片中鸟苷及腺苷的含量。方法:采用高效液相色谱法(HPLC),以水和乙腈为流动相,检测波长为260 nm,体积流量为0.5 ml/min,柱温30℃。结果:通过平行测定,6个批号香云肝泰片均检出鸟苷及腺苷,其鸟苷平均含量为0.1069 mg/g,腺苷平均含量为0.1112 mg/g。结论:本检验方法简便、准确、重现性好,可作为香云肝泰片中主要核苷类成分含量的质量检测方法。
Abstract: Objective: To analyze guanosine and adenosine of Xiangyungantai. Methods: HPLC, with water and acetonitrile as the mobile phase, at 260 nm, volume flow of 0.5 ml/min, column temperature of 30˚C. Results: Guanosine and adenosine were measured in six batches of Xiangyungantai by parallel determination, of which the average content of guanosine is 0.1069 mg/g, and adenosine is 0.1112 mg/g. Conclusion: This test method is simple, accurate, reproducible, and can be used for detecting the main nucleoside content of Xiangyungantai.
文章引用:胡正明, 何银舟, 柴尧2, 嵇晶, 刘睿, 程建明. HPLC法同时测定香云肝泰片中鸟苷和腺苷的含量[J]. 药物资讯, 2017, 6(1): 1-5. https://doi.org/10.12677/PI.2017.61001

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