拟南芥防御相关基因THI2.1启动子与GUS重组载体的构建与转化

Construction of Recombinant Plasmid of Defense-Related Gene THI2.1 Promoter and GUS Gene in Arabidopsis thaliana

摘要: THI2.1基因是植物防御系统中的一个重要基因，其编码的硫菫蛋白能有效的提升植物的抗病性，帮助植物抵御外界病原物的侵染。分析该基因在植物体内的表达模式有利于进一步了解该基因的表达调控。本试验对拟南芥THI2.1基因启动子与带GUS标记基因的载体进行重组构建，并将重组载体转入拟南芥，为后续研究该基因的表达模式提供材料。结果如下：1) 成功的构建了拟南芥THI2.1基因启动子与GUS基因的重组载体。2) 成功的将拟南芥THI2.1基因启动子与GUS基因重组载体转入拟南芥植株，并筛选到了转基因植株。

Abstract: THI2.1 gene is an important gene in plant defense systems; the thionins encoded can effectively enhance plants disease resistance and help plants resist the spread of external pathogens. Analysis of the expression pattern of this gene in plants will help us to further understand the regulation of this gene. In our study, a fusion expression vector of THI2.1 gene promoter and GUS marker gene was constructed and transformed into Arabidopsis thaliana. Our results provide the material basis for further study on the expression pattern of THI2.1 gene in Arabidopsis thaliana. The result as follows: 1) The fusion vector of THI2.1 gene promoter and GUS marker gene was constructed successfully. 2) The reconstructive vector was transferred into Arabidopsis thaliana and the trans- genic plants were screened successfully.