BR  >> Vol. 6 No. 2 (March 2017)

    沙田柚谷胱甘肽转移酶基因(CmGST)的生物信息学及表达分析
    Bioinformatics and Expression Analysis of GST Gene Encoding Glutathione S-Transferase in Citrus maxima cv. Shatian Yu

  • 全文下载: PDF(870KB) HTML   XML   PP.47-54   DOI: 10.12677/BR.2017.62008  
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作者:  

罗琼,肖君,李少梅,龙小霞,刘华英,秦新民:广西师范大学生命科学学院,广西 桂林

关键词:
沙田柚谷胱甘肽转移酶基因生物信息学Citrus maxima cv. Shatian Yu Glutathione S-Transferase Gene Bioinformatics

摘要:
利用高通量测序对沙田柚自交和异交花柱进行转录组测序,通过差异分析得到了沙田柚谷胱甘肽转移酶基因(CmGST)序列。CmGST全长为872 bp (GenBank登录号为KY612456),开放阅读框为657 bp,编码219个氨基酸,推测蛋白质分子量为25.00 kDa,理论等电点pI为5.48。利用生物信息分析软件对CmGST基因进行同源性比对和系统进化分析表,结果显示该基因编码的氨基酸具有谷胱甘肽S转移酶典型的C端与 N端双结构域,属于GST Tau家族蛋白;与克莱门柚(Citrus maxima)的相似度约为98%。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。

In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus maxima cv. Shatian Yu were sequences by high-throughput sequencing technology. The glutathione S-transferase gene (CmGST) sequence of Citrus maxima cv. Shatian Yu was obtained through differential analysis method. The fu11 length of CmGST gene was 872 bp (GenBank accession number: KY612456), and the ORF (Open Reading Frame) length was 657 bp, encoding a protein polypeptide of 219 amino acids with a predicted molecular weight of 25.00 kDa and pI of 5.48. Bioinformatics analysis showed that the CmGST protein contained the Tau GST-specific N-terminal domain (G site) and the C-terminal domain (H site). The homology analysis of amino acid sequence indicated that the glutathione S-transferase protein shared high homology with glutathione S- transferase protein of Citrus clementina (98%). This work provides a systemic sequence analysis of the glutathione S-transferase protein of Citrus maxima cv. Shatian Yu. It will provide the useful reference for further investigation of its structure and function.

文章引用:
罗琼, 肖君, 李少梅, 龙小霞, 刘华英, 秦新民. 沙田柚谷胱甘肽转移酶基因(CmGST)的生物信息学及表达分析[J]. 植物学研究, 2017, 6(2): 47-54. https://doi.org/10.12677/BR.2017.62008

参考文献

[1] Takayama, S. and Isogai, A. (2005) Self-Incompatibility in Plants. Annual Review of Plant Biology, 56, 467-489.
https://doi.org/10.1146/annurev.arplant.56.032604.144249
[2] Lewis, D. (1979) Sexual Incompatibility in Plants. Edward Arnold Limited, London.
[3] Brewbaker, J.L. (1959) Biology of the Angiosperm Pollen Grain. The Indian Journal of Genetics & Plant Breeding, 19, 121-133.
[4] 蓝兴国, 于晓敏, 李玉花. 配子体自交不亲和信号转导的研究进展[J]. 遗传, 2005, 27(4): 677-685.
[5] 安雯, 曹阳, 李俞涛, 等. 植物自交不亲和性的研究进展与展望[J]. 信阳师范学院学报, 2009, 22(4): 630-634.
[6] 胡彬, 蒋建雄, 易自力. 植物配子体自交不亲和机制研究进展[J]. 中国农学通报, 2012, 28(18): 168-173.
[7] 薛妙男, 陈腾士, 杨继华. 沙田柚自交和异交亲和性观察[J]. 园艺学报, 1995, 22(2): 127-132.
[8] 薛妙男, 杨继华. 沙田柚花粉管在花柱中的生长途径及其识别[J]. 广西师范大学学报, 2001, 19(2): 60-66.
[9] 薛妙男, 李义平, 张杏辉, 等. 沙田柚自交花柱S1-RNase的免疫胶体金定位[J]. 广西师范大学学报, 2000, 18(1): 81-84.
[10] 薛妙男, 李义平, 张杏辉, 等. 沙田柚自交花柱中识别蛋白的免疫金定位[J]. 园艺学报, 2001, 28(1): 59-61.
[11] 杨继华, 李红艳, 薛妙男. 沙田柚花柱S-糖蛋白的分离与鉴定[J]. 广西师范大学学报, 2000, 18(4): 66-70.
[12] 杨继华, 尧桂荣, 薛妙男. 沙田柚花柱S-糖蛋白的纯化和N-端序列测定[J]. 广西师范大学学报, 2001, 19(1): 72- 79.
[13] 秦新民, 李惠敏, 薛妙男, 等. 沙田柚自交、异交花粉管蛋白的双向电泳分析[J]. 广西植物, 2004, 24(6): 566-569.
[14] 秦新民, 莫花浓, 万珊, 等. 沙田柚花粉管特异蛋白的免疫细胞化学研究[J]. 广西师范大学学报, 2008, 26(4): 113-115.
[15] 秦新民, 莫花浓, 石菁萍, 等. 沙田柚花粉管S1-RNase免疫胶体金定位研究[J]. 广西农业科学, 2009, 40(5): 483- 485.
[16] Bariola, P.A., Howard, C.J., Taylor, C.B., et al. (1994) The Arabidopsis thaliana Ribonuclease Gene RNS1 Is Tightly Controlled in Response to Phosphate Limitation. The Plant Journal, 6, 673-685.
https://doi.org/10.1046/j.1365-313X.1994.6050673.x
[17] 秦新民, 张渝, 刘玉洁, 等. 沙田柚S-RNase基因的克隆及序列分析[J]. 广西师范大学学报(自然科学版), 2015, 33(1): 139-145.
[18] Dixon, D.P., Davis, B.G. and Edwards, R. (2002) Functional Divergence in the Glutathione Transferase Superfamily in Plants. Journal of Biological Chemistry, 277, 30859-30869.
https://doi.org/10.1074/jbc.M202919200
[19] Moons, A. (2005) Regulatory and Functional Interactions of Plant Growth Regulators and Plant Glutathione S-Transferases (GSTs). Vitamins & Hormones, 72, 155-202.
[20] 胡廷章, 周大祥, 罗凯. 植物谷胱甘肽转移酶的结构与功能及其基因表达[J]. 植物生理学通讯, 2007, 43(1): 195- 200.
[21] Grotewold, E. (2001) Subcellular Trafficking of Phytochemicals. Recent Research Developments in Plant Physiology, 2, 31-48.
[22] Alfenito, M.R., Souer, E., Goodman, C.D., et al. (1998) Functional Complementation of Anthoeyanin Sequestration in the Vacuole by Widely Divergent Glutathiones Transferases. Plant Cell, 10, 1135-1150.