BR  >> Vol. 6 No. 2 (March 2017)

    棕榈藤不同部位中多糖、多酚、黄酮类化合物的分布研究
    Study on the Distribution of Polysaccharide, Polyphenols, and Flavonoids from Different Organs of Rattan

  • 全文下载: PDF(695KB) HTML   XML   PP.63-70   DOI: 10.12677/BR.2017.62010  
  • 下载量: 333  浏览量: 811   国家科技经费支持

作者:  

曹琴,孙殷楠,杨奎,孙晋德,马春慧:东北林业大学材料科学与工程学院,黑龙江 哈尔滨

关键词:
棕榈藤多糖多酚黄酮匀浆Rattan (Palm) Polysaccharides Polyphenols Flavonoids Homogenate

摘要:
本研究以棕榈藤为原料,对其各部位(包括棕榈藤果皮与果肉、棕榈藤嫩茎、棕榈藤叶鞘和叶片、棕榈藤藤茎)进行匀浆辅助提取后,分别采用水加热搅拌提取和80%乙醇回流提取,然后对水提取物和醇提取物中的多糖、多酚及黄酮类化合物进行测定,从而研究多糖、多酚及黄酮类化合物在棕榈藤植物的不同部位中的分布情况。本文还对匀浆辅助萃取的机理进行了阐释。实验结果表明:糖类化合物多分布于果实与嫩茎中,果皮与果肉中含多糖29.31%和7.27%,嫩茎中含多糖12.14%,叶鞘、叶片和藤茎中的多糖含量分别为2.91%、1.87%和1.99%。多酚和黄酮类化合物多分布于果实中,果皮(多酚5.33%,黄酮4.67%)略高于果肉(多酚5.22%,黄酮4.64%),其次是叶片(多酚3.35%,黄酮2.92%)、叶鞘(多酚2.68%,黄酮2.37%)和嫩茎(多酚1.97%,黄酮1.83%),藤茎中含量最少(多酚0.55%,黄酮0.47%)。

In this study, Rattan (palm cane) as the raw material, the different parts of Rattan plant were extracted by homogenate-assisted extraction, and then extracted by water heating stirring extraction and reflux extraction with 80% ethanol, respectively. The extraction yields of aqueous extractives and alcohol extractives from different parts of Rattan, and polysaccharides, polyphenols and flavonoids content in aqueous extractives and alcohol extractives were determined. And the details of the homogenate-assisted extraction mechanism were interpreted. The experimental results showed that the polysaccharide content in the pericarp (29.31%) and sarcocarp (7.27%) was the highest, and in the tender stems (12.14%) was less, and in sheath, blade, and rattan stem were 2.91%, 1.87% and 1.99%, respectively. However, the polyphenols and flavonoids content in alcohol extractives was higher, which in the pericarp (polyphenols 5.33%, flavonoids 4.67%) and sarcocarp (polyphenols 5.22%, flavonoids 4.64%) was the highest, and followed by blade (polyphenols 3.35%, flavonoids 2.92%), sheath (polyphenols 2.68%, flavonoids 2.37%), and tender stems (polyphenols 1.97%, flavonoids 1.83%), and in the rattan stem (polyphenols 0.55%, flavonoids 0.47%) was the least.

文章引用:
曹琴, 孙殷楠, 杨奎, 孙晋德, 马春慧. 棕榈藤不同部位中多糖、多酚、黄酮类化合物的分布研究[J]. 植物学研究, 2017, 6(2): 63-70. https://doi.org/10.12677/BR.2017.62010

参考文献

[1] Mohan Ram, H.Y. and Tandon, R. (1997) Bamboo and Rattans: From Riches to Rags. Proceedings of the Indian National Science Academy, 63, 245-267.
[2] 钟如松, 何洁英, 伍有声, 等. 引种棕榈图谱[M]. 合肥: 安徽科学技术出版社, 2004: 355.
[3] 林秀香, 陈振东. 我国棕榈科植物的研究进展[J]. 热带作物学报, 2007, 28(3): 115-119.
[4] 竺肇华. 中国热带地区竹藤发展[M]. 北京: 中国林业出版社, 2001.
[5] 许煌灿, 尹光天, 孙清鹏, 等. 棕榈藤的研究和发展[J]. 林业科学, 2002, 38(2): 135-143.
[6] 李时珍. 本草纲目[M]. 北京: 人民卫生出版社, 1977: 750-751.
[7] 刘善庭, 李建美, 王清, 辛勤, 林丽文, 李军, 于江, 杨静, 梁玉记, 齐汝霞. 棕榈果实水–醇提取物的药理研究[J]. 济宁医学院学报, 2003, 26(1): 36-37.
[8] 王艳, 茹仙古丽·哈斯木, 韩艳春, 阿孜古丽·伊明, 阿依吐伦·斯马义. 苯酚-硫酸法测定维吾尔药核桃分心木多糖的含量[J]. 亚太传统医药, 2012, 8(2): 35-37.
[9] 詹莉莉, 章程辉, 常刚, 叶俊松, 潘晓威. 福林酚比色法测定露兜簕果实中的总多酚[J]. 广东农业科学, 2013(4): 77-80.
[10] 钟文, 翟瑶, 卢永昌. 藏药宽筋藤中总黄酮和芦丁含量的测定[J]. 湖北农业科学, 2013(9): 2151-2170.
[11] 祖元刚, 赵春建, 李春英, 史权, 焦琰. 鲜法匀浆萃取烟叶中茄尼醇的研究[J]. 高校化学工程学报, 2005, 19(6): 757-761.
[12] 刘晓芳, 刘满红, 张荣来, 黄桂文. 双水相–匀浆萃取法提取芒果核多酚工艺研究[J]. 云南民族大学学报: 自然科学版, 2015, 24(2): 144-146.
[13] 史权. 鲜芽匀浆空化高效提取10-羟基喜树碱和喜树碱创新技术研究[D]: [博士学位论文]. 哈尔滨: 东北林业大学, 2004.