肝癌血清中MFG-E8水平及其在肝癌转移作用研究
The Level of Serum MFG-E8 in Liver Cancer and Its Metastasis in Liver Cancer
DOI: 10.12677/WJCR.2017.72007, PDF, HTML, XML, 下载: 1,969  浏览: 4,393 
作者: 洪宏海, 王征, 李磊, 林丽英, 夏勇, 刘美玲：广州医科大学附属第三医院检验科，广东 广州；苏宝昌：暨南大学附属华侨医院血库，广东 广州
关键词: 肝癌；MFG-E8；肿瘤转移；Hepatocellular Carcinoma； MFG-E8； Cancer metastasis
摘要: 目的：检测肝癌病人血清中MFG-E8水平及探讨其在肝癌中作用。方法：ELISA法检测血清中MFG-E8水平；分子克隆构建MFG-E8真核表达载体；MTT法和Migration assay法分别检测肝癌增殖和转移作用。结果：肝癌病人血清中MFG-E8水平明显升高，对肝癌诊断具有很好的价值，且和AFP水平呈正相关；过表达MFG-E8促进肝癌转移。结论：肝癌病人血清MFG-E8水平升高，其促进肝癌转移，对肝癌诊断具有很好价值。
Abstract: Objective: to detect the level of MFG-E8 in serum of patients with liver cancer and its role in liver cancer. Methods: ELISA was used to detect the level of MFG-E8 in serum. Molecular cloning was used to construct MFG-E8 eukaryotic expression vector. MTT assay and Migration assay were used to detect the proliferation and metastasis of HCC. Results: the level of MFG-E8 in patients’ serum with liver cancer was significantly increased, which has a good value in the diagnosis of liver cancer, and is positively correlated with the level of AFP. Overexpression of MFG-E8 can promote the metastasis of liver cancer. Conclusion: the level of serum MFG-E8 in patients with liver cancer is high, and it can promote the metastasis of liver cancer.
文章引用：洪宏海, 王征, 李磊, 林丽英, 夏勇, 刘美玲, 苏宝昌. 肝癌血清中MFG-E8水平及其在肝癌转移作用研究[J]. 世界肿瘤研究, 2017, 7(2): 40-47. https://doi.org/10.12677/WJCR.2017.72007

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