动物源粪肠球菌的遗传关系分析
MLST Analysis of Different Sources of Enterococcus Faecalis
DOI: 10.12677/HJAS.2017.72017, PDF, HTML, XML, 下载: 1,508  浏览: 4,948 
作者: 赵 娣, 崔新格, 闫瑞杰, 陈 飞, 李滨洲, 胡 慧, 王亚宾*：河南农业大学牧医工程学院，河南 郑州；段志刚：郑州市畜牧局，河南 郑州
关键词: 粪肠球菌；多位点序列分析；管家基因；序列型(ST)；Enterococcus Faecalis； Multilocus Sequence Typing (MLST)； Housekeeping Gene； Sequence Type (ST)
摘要: 目的：为了探讨不同动物来源中粪肠球菌(E.faecais)菌株之间的遗传关系。方法：利用PCR技术对59株不同来源粪肠球菌的7个管家基因gdh、gyd、pstS、gki、aroE、xpt、yiqL分别进行扩增测序，根据测序结果，利用多位点序列分型技术(Multilocus Sequence Typing, MLST)进行分析、比对。结果：59株不同来源粪肠球菌被分成了27个序列型(Sequence type, ST)，其中，主要流行序列型是ST-16 (占16.9%)，其次是ST-163 (占6.8%)、ST-238 (占6.8%)和ST-631 (占6.8%)，而与医院内感染密切相关的ST-16序列型，广泛分布在除熟食食品和羊粪外的其它5种来源中。另外，猪和蜂猴的粪便中分别含有7种序列型，且均有ST-16序列型。结论：多种动物来源中均含有感染人的粪肠球菌序列型，这也为粪肠球菌致病机理研究奠定了基础。
Abstract: Objective: To explore the genetic relationships of E.faecais originating from different animal origions. Methods :The seven housekeeping genes including gdh, gyd, pstS, gki, aroE, xpt and yiqL of all 59 E.faecais originating from different animal origions were amplified by PCR method and sequenced, respectively. The sequences were analyzed by multilocus sequence typing technique (Multilocus Sequence Typing, MLST). Results: The 59 E.faecais were divided into 27 sequences type (Sequence type, ST), among them, the major epidemic sequence was ST-16 (16.9%), followed byST-163 (representing 6.8%), ST-238 (6.8%) and ST-631 (6.8%), and is closely related to nosocomial infection of ST-16 sequence type, the ST-16 Sequence type associated with clinical infection were widely distributed in slow loris, chicken-origin, lesion viscera, fresh pork and pig dung. Additionally, seven sequences type were widely distributed in the pig and slow loris stool. And, all of them have ST-16 sequence type. Conclusion: A variety of animal sources contain the dung enterococcus sequence of infected people, it also laid the basis for study of pathogenesis in E. faecalis.
文章引用：赵娣, 段志刚, 崔新格, 闫瑞杰, 陈飞, 李滨洲, 胡慧, 王亚宾. 动物源粪肠球菌的遗传关系分析[J]. 农业科学, 2017, 7(2): 140-146. https://doi.org/10.12677/HJAS.2017.72017

参考文献

[1] 卢雅敏. 肠球菌毒力基因和万古霉素耐药基因研究[D]: [硕士学位论文]. 温州: 温州医学院, 2013: 1-57.
[2] 刘磊, 王亚宾, 程金乎, 等. 猪粪肠球菌两种致病基因和表型的检测[J]. 中国农学通报, 2009, 25(1): 17-20.
[3] 徐建国. 临床分离肠球菌耐药性、毒力基因及多位点序列分型研究[D]: [硕士学位论文]. 杭州: 浙江大学, 2010: 1-59.
[4] Shanker, N., Lockatell, C.V. and Baghdayan, A.S. (2001) Role of Enterococcus faecium Surface Protein Esp in the Pathogenesis of Ascending Urinary Tract Infection. Infection and Immunity, 69, 4366-4372.
https://doi.org/10.1128/IAI.69.7.4366-4372.2001
[5] Van, D., Bogaard, A.E., Jansen, L.B., et al. (1997) Vancomycin-Resistant Enterococci in Turkeys and Farmers. New England Journal of Medicine, 337, 1558-1559.
https://doi.org/10.1056/NEJM199711203372117
[6] 姬小薇, 廖亚玲, 毛旭虎, 等. MLST分析在病原微生物基因分型应用中的研究进展[J]. 国际检验医学杂志, 2011, 32(2): 246-249.
[7] Cooper, J.E. and Feil, E.J. (2004) Multilocus Sequence Typing What Is Resolved. Trends in Microbiology, 12, 373- 377.
https://doi.org/10.1016/j.tim.2004.06.003
[8] 王浩竹, 王正祥. 细菌分子鉴定技术的研究进展[J]. 安徽农业科学, 2008, 36(23): 9880-9884.
[9] 王中强, 邱少富, 王勇, 等. 多位点序列分型技术及其研究进展[J]. 军事医学科学院刊, 2010, 34(1): 76-79.
[10] 赵冰, 朱林英, 黄红, 等. 多位点序列分析在空肠弯曲菌监测中的运用[J]. 职业与健康, 2016, 32(20): 2792-2795.
[11] 赵瑞珂, 顾国浩, 韩清珍, 等. 褪色沙雷菌耐药率与多位点序列分析研究[J]. 中华医院感染学杂志, 2016, 26(18): 4084-4087.
[12] Klein-Jobstl, D., Sofka, D., Iwersen, M., et al. (2016) Multilocus Sequence Typing and Antimicrobial Resistance of Campylobacter jejuni Isolated from Dairy Calves in Austria. Frontiers in Microbiology Trends in Microbiology, 7, 72.
https://doi.org/10.3389/fmicb.2016.00072
[13] Korczak, B.M., Zurfluh, M., Emler, S., et al. (2009) Multiplex Strategy for Multilocus Sequence Typing, Fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland. Journal of Clinical Microbiology, 47, 1996-2007.
https://doi.org/10.1128/JCM.00237-09
[14] 王中强, 邱少富, 王勇, 等. 多位点序列分型技术及其研究进展[J]. 军事医学科学院刊, 2010, 34(1): 76-79.
[15] Nallaparedy, S.R., Wenxiang H., Weinstock, G.M., et al. (2005) Molecular Characterizations of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island. J. Bacterio, 187, 5709-5718.
https://doi.org/10.1128/jb.187.16.5709-5718.2005
[16] 王亚宾. 猪肠球菌病病原分离鉴定及其分子致病机制研究[D]: [博士学位论文]. 郑州: 河南农业大学, 2009: 1-116.
[17] Quinones, D., Kobayashi, N. and Nagashima, S. (2009) Molecular Epidemiologic Analysis of Enterococcus faecalisisolates in Cuba by Multilocus Sequence Typing. Microbial Drug Resistance, 15, 287-293.
https://doi.org/10.1089/mdr.2009.0028
[18] Poulsen, L.L., Bisgaard, M., Son, N.T., et al. (2012) Enterococcus and Streptococcus spp. Associated with Chronic and Self-Medicated Urinary Tract Infections in Vietnam. BMC Infectious Diseases, 12, 320-327.
https://doi.org/10.1186/1471-2334-12-320
[19] Vergis, E.N., Shankar, N., Chow, J.W., et al. (2002) Association between the Presence of Enterococcal Virulence Factors Gelatinase, Hemolysin, and Enterococcal Surface Protein and Mortality among Patients with Bacteremia Due to Enterococcus faecalis. Clinical Infectious Diseases, 35, 570-575.
https://doi.org/10.1086/341977

为你推荐