鸡缺硒心肌损伤代谢组学分析
Metabonomics Analysis of Myocardial Injury Induced by Selenium Deficiency in Chicken
DOI: 10.12677/OJNS.2017.52018, PDF, HTML, XML, 下载: 1,687  浏览: 6,056  科研立项经费支持
作者: 刘 伟：东北农业大学动物医学学院内科教研室，黑龙江 哈尔滨；哈尔滨医科大学附属第二医院心肌缺血教育部重点实验室，黑龙江 哈尔滨；姚海东, 赵文超, 徐世文*：东北农业大学动物医学学院内科教研室，黑龙江 哈尔滨；孙 萌：哈尔滨医科大学附属第二医院心肌缺血教育部重点实验室，黑龙江 哈尔滨
关键词: 鸡心肌细胞；缺硒；代谢组；Chicken Myocardial Cell； Selenium Deficiency； Metabonomics
摘要: 目的：探讨缺硒致鸡心肌损伤的代谢组学变化。方法：选用1日龄雏鸡，采用低硒饲料喂养建立鸡缺硒心肌损伤模型；通过血浆代谢组学方法检测差异代谢物质。结果：发现正负离子状态下，缺硒组血浆均有显著差异代谢物质，共计16种。其中显著上调表达的有胆碱、磷脂酰甘油、和亮氨酸(P < 0.01)。结论：在缺硒组中观察到甘油、磷脂、亮氨酸、类固醇等生化物质的异常代谢。这些潜在的生物标志物与炎症、凋亡、和血小板聚集有关。可初步为深入理解鸡缺硒心肌损伤代谢机制提供新的信息。
Abstract: Research purposes: Study on the changes of myocardial injury induced by selenium deficiency in chicken. Methods: 1-day old chicks with low selenium feed were chosen to build the chicken selenium deficient myocardial injury model. Plasma metabonomics analysis was applied to detect the differential metabolites. Results: There were 16 differential metabolites in selenium deficient plasma in positive and negative ion state. Among them, the abundance of choline, phosphatidyl glycerol and leucine were up-regulated (P < 0.01). Conclusion: Abnormal metabolism of glycerol and phospholipids, leucine, steroids and other biochemical substances were found in selenium deficiency group. These potential biomarkers are associated with inflammation, apoptosis, and platelet aggregation. It can provide new information for understanding the metabolic mechanism of selenium deficiency in chicken.
文章引用：刘伟, 姚海东, 赵文超, 孙萌, 徐世文. 鸡缺硒心肌损伤代谢组学分析[J]. 自然科学, 2017, 5(2): 126-136. https://doi.org/10.12677/OJNS.2017.52018

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