BR  >> Vol. 6 No. 3 (May 2017)

    油菜素内酯对桔梗组培幼苗生长和生理特性的影响
    The Effects of Brassinosteroid on Growth and Physiological Properties of Platycodon grandiflorum Seedlings in Tissue Culture

  • 全文下载: PDF(538KB) HTML   XML   PP.134-138   DOI: 10.12677/BR.2017.63018  
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作者:  

罗明华:绵阳师范学院生命科学与技术学院,四川 绵阳;生态安全与保护四川省重点实验室,四川 绵阳;
康卫龙,杨迪,邹利娟:绵阳师范学院生命科学与技术学院,四川 绵阳;
顾冰,敬章书:四川同兴天然植物药业有限公司,四川 中江

关键词:
桔梗幼苗油菜素内酯生长生理特性Seedlings of Platycodon grandiflorum Brassinosteroid Growth Physiological Properties

摘要:
本文研究了生根培养基中添加不同浓度的油菜素内酯(BR)对桔梗组培幼苗生长和生理特性的影响,外源BR的浓度为0, 0.025, 0.05, 0.10, 0.20, 0.40 mg/L。结果表明:幼苗不定根的条数、幼苗节数、叶绿体色素含量、POD和SOD活性均随BR浓度的增加呈先增高后降低趋势,在0.10 mg/L处理达到最大值,说明该处理有利于培养健壮幼苗,提高叶绿素含量和增强幼苗的抗性。

In this study, the effects of Brassinosteriod (BR) on growth and physiological properties of Platycodon grandiflorum seedlings were conducted by adding exogenous BR in the root inducing medium, and the concentration of exogenous BR is 0, 0.025, 0.05, 0.10, 0.20, 0.40 mg/L. The results showed that the number of adventitious root and node, chlorophyll content, activity of POD and SOD in P. grandiflorum seedlings were increased first and then decreased, and the maximum value in 0.1 mg/L treatment, respectively. Obviously, BR application could significantly increase the chlorophyll content, the resistance of the P. grandiflorum leaves, and cultivate the health and strong seedlings in the 0.1 mg/L treatment. The results provided theoretical foundation in seedling culture of P. grandiflorum.

文章引用:
罗明华, 康卫龙, 杨迪, 顾冰, 邹利娟, 敬章书. 油菜素内酯对桔梗组培幼苗生长和生理特性的影响[J]. 植物学研究, 2017, 6(3): 134-138. https://doi.org/10.12677/BR.2017.63018

参考文献

[1] Ma, X., Bucalo, K., Deternann, R., et al. (2012) Somatic Embryo Genesis Ptant Regeneration and Cryopreseration for Torreya Taxifolia a Highly Endangered Coniferous Species. In Vitro Cell Dev Biol Plant, 48, 324-334.
https://doi.org/10.1007/s11627-012-9433-4
[2] 闫慧芳, 曾炳山, 范春节, 等. 油菜素内酯对巨桉组培中不定根诱导、苗木生长和茎基部细胞结构的影响[J]. 广西植物, 2016, 6(7): 763­767.
[3] 束红梅, 倪万潮, 郭书巧. 油菜素内酯代谢相关基因及其调控植物耐盐性的研究进展[J]. 分子植物育种, 2011, 9(1): 261-266.
[4] 钟妍婷, 原向阳, 刘哲, 等. 油菜素内酯处理对谷子农艺性状和生理特性的影响[J]. 作物杂志, 2015(2): 124-128.
[5] 国家药典委员会. 中华人民共和国药典(一部)(2015版)[M]. 北京: 中国医药科技出版社, 2015: 277.
[6] 李合生. 植物生理生化实验原理与技术[M]. 北京: 高等教育出版社, 2003: 135.
[7] Omran, R.G. (1980) Peroxide Levels and Activities of Catalase, Peroxidase and Indoleacetic Acid Oxidase during and after Chilling Cucumber Seedlings. Journal of Plant Physiology, 65, 407-408.
https://doi.org/10.1104/pp.65.2.407
[8] 邵从本, 罗广华, 王爱国. 几种检测超氧化物岐化酶活性反应的比较[J]. 植物生理学通讯, 1983, 19(5): 46-49.
[9] Krishna, P. (2003) Brassinosteroid-Mediated Stress Responses. Journal of Plant Growth Regulation, 22, 289-297.
https://doi.org/10.1007/s00344-003-0058-z
[10] Haubrick, L.L. and Assmann, S.M. (2006) Brassinosteroids and Plant Function: Some Clues, More Puzzles. Plant, Cell and Environment, 29, 446-457.
https://doi.org/10.1111/j.1365-3040.2005.01481.x
[11] 侯雷平, 李梅兰. 油菜素内酯(BR)促进植物生长机理研究进展[J]. 植物学通报, 2001, 18(5): 560-566.
[12] 李启任, 王小柱, 杜嵩生. 表高油菜素内酯在满天星组培中的应用[J]. 云南大学学报(自然科学版), 1999, 21(5): 413-415.
[13] 张琳. 油菜素内酯的生理效应及发展前景[J]. 北方园艺, 2011, 20: 188-191.
[14] 阮英慧, 董守坤, 刘丽君, 等. 干旱胁迫下油菜素内酯对大豆花期生理特性的影响[J]. 作物杂志, 2011(6): 33-37.