BR  >> Vol. 6 No. 3 (May 2017)

    运用SCAR和CAPS分子标记辅助筛选辣椒恢复系研究
    Using the SCAR and CAPS Markers Assisted Selection Pepper Restorer Research

  • 全文下载: PDF(1252KB) HTML   XML   PP.158-166   DOI: 10.12677/BR.2017.63021  
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作者:  

张洪源,张敏,谈杰,黄树苹,王春丽,陈霞,谈太明:武汉市农业科学技术研究院蔬菜科学研究所,湖北 武汉

关键词:
辣椒恢复基因SCARCAPS分子标记Pepper (Capsicum annuum L.) Rf Gene Sequence-Characterized Amplified Regions (SCAR) Cleaved Amplified Polymorphic Sequence (CAPS) Molecular Maker

摘要:
以辣椒(Capsicum annuum L.)幼嫩叶片为研究材料,利用3对显性SCAR标记和1对共显性CAPS标记对16份已知育性的辣椒材料进行引物可行性验证,再利用这4对引物对112份未知恢复基因资源的辣椒材料进行恢复基因鉴定。结果表明:SCAR和CAPS在16份辣椒材料中的结果完全一致,112份未知恢复基因辣椒资源鉴定中有104份材料在4对引物检测结果完全一致,其中51份具有恢复基因连锁的特异带型,占比49.04%,且22份具有纯合的恢复基因。本研究将SCAR和CAPS分子标记结合起来建立了与恢复基因Rf有关的PCR检测方法,可对辣椒恢复基因及其等位基因纯合状态进行分子标记辅助筛选。

Pepper (Capsicum annuum L.) tender leaves were used as the research object in this present study. Three pairs of dominant SCAR markers and a pair of co-dominant CAPS markers were applied to detect sixteenth known sterility pepper materials for verifying the feasibility of these primers. Then, the four pairs of primers were used to identify restorer genes in one hundred and twelve unknown pepper materials. The result showed that SCAR and CAPS displayed completely consistent in the sixteenth pepper materials, which were known contain or uncertain restorer gene peppers. In the one hundred and twelve unknown restorer genes pepper resources, one hundred and four materials were identical in the four pairs of primers detection results, including fifty-one had dominant restorer genes, accounted for 49.04%, and twenty-two materials had homozygous restoring genes. SCAR and CAPS molecular markers were combined and established a restorer gene PCR detection method in this present study, which can be used in marker-assisted selection restoring genes and alleles homozygous state.

文章引用:
张洪源, 张敏, 谈杰, 黄树苹, 王春丽, 陈霞, 谈太明. 运用SCAR和CAPS分子标记辅助筛选辣椒恢复系研究[J]. 植物学研究, 2017, 6(3): 158-166. https://doi.org/10.12677/BR.2017.63021

参考文献

[1] Martin, J. and Crawford, J.H. (1951) Several Types of Sterility I Capsicum Frutescens. Proceedings of the American Society of Horticultural Science, 57, 335-338.
[2] Peterson, P.A. (1958) Cytoplasmically Inherited Male Sterility in Capsicum. American Naturalist, 92, 111-119.
https://doi.org/10.1086/282017
[3] Chen, S. (1997) Male Sterility in Pepper (Capsicum annuum L.). Euphytica, 93, 83-88.
https://doi.org/10.1023/A:1002947907046
[4] 马越, 黄炜, 吉姣姣, 巩振辉, 尹川川. 几个辣椒核质互作雄性不育材料的育性调查[J]. 北方园艺, 2012(11): 5-7.
[5] 徐小万, 黄旺平, 吴智明, 李涛, 李颖, 王恒明. 应用分子标记筛选辣椒雄性不育恢复系研究[J]. 广东农业科学, 2015, 42(23): 139-144.
[6] 杨娟, 王雯, 沈火林. 辣椒恢复基因SSR标记定位及分子标记辅助选择育种[J]. 中国瓜菜, 2010, 23(5): 1-5.
[7] Kim, H.J., Baek, K.H., Lee, S.W., Kim, J., Lee, B.W., Cho, H.S., Kim, W.T., Choi, D. and Hur, C.G. (2008) Pepper EST Database: Comprehensive in Silico Tool for Analyzing the Chili Pepper (Capsicum annuum) Transcriptome. BMC Plant Biology, 8, 101.
https://doi.org/10.1186/1471-2229-8-101
[8] Li, J., Pandeya, D., Jo, Y.D., Liu, W.Y. and Kang, B.C. (2013) Reduced Activity of ATP Synthase in Mitochondria Causes Cytoplasmic Male Sterility in Chili Pepper. Planta, 237, 1097-1109.
https://doi.org/10.1007/s00425-012-1824-6
[9] Jo, Y.D., Ha, Y., Lee, J.H., Park, M., Bergsma, A.C., Choi, H., et al. (2016) Fine Mapping of Restorer-of-Fertility in Pepper (Capsicum L.) Identified a Candidate Gene Encoding a Pentatricopept Repeat (PPR)-Containing Protein. Theoretical and Applied Genetics, 129, 2003-2017.
https://doi.org/10.1007/s00122-016-2755-6
[10] Jo, Y.D., Kim, Y.M., Park, M.N., Yoo, J.H., Park, M.K., Kim, B.D. and Kang, B.C. (2010) Development and Evaluation of Broadly Applicable Markers for Restorer-of-Fertility in Pepper. Molecular Breeding, 25, 187-201.
https://doi.org/10.1007/s11032-009-9318-3
[11] 沈火林, 蒋健箴, 王志源, 等. 辣椒雄性不育系选育及遗传研究[J]. 北京农业大学学报, 1994, 20(1): 25-30.