截形十二卷组培快繁试验

Haworthia Tissue Cultivate and Experimental Research

摘要: 以玉扇多肉为接种外植体，对其组织培养技术进行了初步的研究。结果表明：MS + 1.0 mg∙L⁻¹ 6-BA + 0.4 mg∙L⁻¹ NAA诱导效果最好，诱导率可达97%；MS + 2.0 mg∙L⁻¹ IAA + 2.5 mg∙L⁻¹ 6-BA + 0.25 mg∙L⁻¹ NAA增殖效果最好，培养40 d后增殖率可达3.6倍；1/2MS + 0.01 mg∙L⁻¹ IAA + 0.05 mg∙L⁻¹ NAA生根效果最好，且植株长势健壮。炼苗基质以草炭土:珍珠岩:蛭石(1:1:1)为最好；炼苗温度23˚C~28˚C，空气相对湿度80%~90%有利于提高炼苗成活率。

Abstract: The technique of tissue culture was studied in this paper. The results show that MS + 1.0 mg∙L⁻¹ 6-BA + 0.4 mg∙L⁻¹ NAA is best induced effect. Induction rate could reach 97%. MS + 2.0 mg∙L⁻¹ IAA + 2.5 mg∙L⁻¹ 6-BA + 0.25 mg∙L⁻¹ NAA is induced proliferation of the best effect. Proliferation rate can be up to 3.6 times. 1/2MS + 0.01 mg∙L⁻¹ IAA + 0.05 mg∙L⁻¹ NAA is the best to take root and plants grow strong. The seeding matrix is the best in the soil of turf:perlite:vermiculite (1:1:1), smelting plants 23˚C - 28˚C temperature, air relative humidity of 80% - 90% is helpful to improve the survival rate of seedlings.