植物病害生防菌株的研究进展
Research Development of Microbial Antagonists against Plant Disease
DOI: 10.12677/AMB.2017.62005, PDF, HTML, XML,  被引量 下载: 1,970  浏览: 5,546  国家自然科学基金支持
作者: 陈嘉敏, 朱洁倩:湖北工程学院,生命科学技术学院/特色果蔬质量安全控制湖北省重点实验室,湖北 孝感;湖北大学,生命科学学院,湖北 武汉;管维轩:湖北工程学院,化学与材料科学学院,湖北 孝感;魏 蜜*, 傅本重, 李国元:湖北工程学院,生命科学技术学院/特色果蔬质量安全控制湖北省重点实验室,湖北 孝感
关键词: 生防菌株来源种类抑菌物质生防机制Microbial Biocontrol Origin Species Active Substance Biocontrol Mechanism
摘要: 生防菌株资源丰富,具有对环境友好的优点,在植物病害绿色防治上具有重要作用。本文简要介绍了近年来植物病害通过生防菌进行生物防治的国内外研究进展,包括生防菌株的来源、种类、抑菌物质以及对植物病害的生防机制,提出了对生防菌株进行基因改造、诱变育种和菌剂复配可能成为今后技术研究的重点。
Abstract: The microbial biocontrol agents are rich in natural resources, and ecofriendly in application, it is an important factor in plant diseases management. The recent development of microbial antagonists against plant disease pathogens were reviewed in this paper, including the origins, species, and antimicrobial substances of the microbes, as well as the biocontrol mechanisms were discussed. Furthermore, we afforded that the genetic breeding and modification, and multiple microbes, would be the prospective techniques to improving the efficiency of them in research and application in the future.
文章引用:陈嘉敏, 管维轩, 朱洁倩, 魏蜜, 傅本重, 李国元. 植物病害生防菌株的研究进展[J]. 微生物前沿, 2017, 6(2): 35-43. https://doi.org/10.12677/AMB.2017.62005

参考文献

[1] 刘畅, 许家来, 郭凯, 等. 烟草黑胫病生防菌的筛选鉴定及发酵条件优化[J]. 江苏农业科学, 2016, 45(5): 167- 170.
[2] 李宛泽, 郭月霞, 王忠武. 玉米茎腐病生防菌的筛选鉴定及最佳培养条件研究[J]. 江苏农业科学, 2016, 44(3): 148-149.
[3] Chen, F., Wang, M., Zheng, Y., et al. (2010) Quantitative Changes of Plant Defense Enzymes and Phytohormone in Biocontrol of Cucumber Fusarium Wilt by Bacillus Subtilis, B579. World Journal of Microbiology and Biotechnology, 26, 675-684.
https://doi.org/10.1007/s11274-009-0222-0
[4] Ma, Y., Tan, X., Liu, J., et al. (2015) Identification of an Endophytic Biocontrol Strain NS03 and Its Efficacy in Controlling Pomegranate Dry Fruit Rot Disease. Acta Horticulturae, 1089, 145-151.
[5] Hahm, S.S., Kim, J.T., Han, K.S., et al. (2012) Biocontrol Efficacy of Endophytic Bacteria Flavobacterium hercynim EPB-C313 for Control of Chinese Cabbage Clubroot. Research in Plant Disease, 18, 210-216.
https://doi.org/10.5423/RPD.2012.18.3.210
[6] Cheah, L.H., Page, B.B.C., Cheah, L.H., et al. (1997) Trichoderma SPP. for Potential Biocontrol of Clubroot of Vegetable Brassicas. Proceedings of the New Zealand Plant Protection Conference. Christchurch, 11-13 August 1997, 150- 153.
[7] 苏婷, 路梅, 周青, 等. 抗不同生化型青枯菌的生防茵筛选鉴定及其活性分析[J]. 植物保护学报, 2010, 37(5): 431-435.
[8] Okazaki, T. and Okami, Y. (1972) Studies on Marine Microorganisms. II. Actinomycetes in Sagami Bay and Their Antibiotic Substances. Journal of Antibiotics, 25, 461-466.
https://doi.org/10.7164/antibiotics.25.461
[9] 谢永丽, 马莉贞, 徐志伟, 等. 冻土荒漠区分离低温适生PGPR菌的鉴定及其抗菌促生特性[J]. 中国生物防治学报, 2014, 30(1): 94-100.
[10] Kloepper, J.W., Leong, J., Teintze, M., et al. (1980) Enhanced Plant Growth by Siderophores Produced by Plant Growth-Promoting Rhizobacteria. Nature, 286, 885-886.
https://doi.org/10.1038/286885a0
[11] 赵培静, 任文彬, 缪承杜, 等. 淡紫拟青霉研究进展与展望[J]. 安徽农业科学, 2007, 35(30): 9672-9674.
[12] 王昌家, 宋超英, 张新德, 等. 淡紫拟青霉菌料防治大豆胞囊线虫的后效研究[J]. 中国生物防治学报, 1997, 13(1): 26-28.
[13] 陈捷, 窦恺, 高永东, 等. 木霉菌在玉米病害生物防治中的作用机制及应用(英文)[J]. 菌物学报, 2014, 33(6): 1154-1167.
[14] 许秀兰, 黄晓丽, 张翅, 等. 云杉内生优势毛壳菌的筛选及其生防机制研究[J]. 中国生物防治学报, 2014, 30(4): 511-519.
[15] 林茂松, 沈素文. 厚壁孢子轮枝菌防治南方根结线虫研究初报[J]. 中国生物防治学报, 1994, 10(1): 7-10.
[16] 聂海珍, 孙漫红, 李世东, 等. 棉隆与淡紫拟青霉联合防治番茄根结线虫病的效果评价[J]. 植物保护学报, 2016, 43(4): 689-696.
[17] 范青, 田世平, 徐勇. 丝孢酵母对苹果采后灰霉病和青霉病抑制效果的影响[J]. 中国农业科学, 2001, 34(2): 163-168.
[18] 程根武, 王勇, 杨秀荣, 等. 拮抗酵母菌用于产后病害生物防治的研究进展[J]. 天津农业科学, 2002, 8(1): 44-46.
[19] Vos, C., Claerhout, S., Mkandawire, R., et al. (2012) Arbuscular Mycorrhizal Fungi Reduce Root-Knot Nematode Penetration through Altered Root Exudation of Their Host. Plant & Soil, 354, 335-345.
https://doi.org/10.1007/s11104-011-1070-x
[20] Kamińska, M., et al. (2010) Effect of Arbuscular Mycorrhizal Fungi Inoculation on Aster Yellows Phytoplasma-In- fected Tobacco Plants. Scientia Horticulturae, 125, 500-503.
https://doi.org/10.1016/j.scienta.2010.04.027
[21] 郭珺, 武爱莲, 闫敏, 等. 芽孢杆菌Pb-4菌株鉴定及其抑菌活性的研究[J]. 华北农学报, 2016, 31(2): 224-230.
[22] 严婉荣, 赵廷昌, 肖彤斌, 等. 生防细菌在植物病害防治中的应用[J]. 基因组学与应用生物学, 2013(4): 533-539.
[23] 常琳, 肖琦, 童蕴慧, 等. gacS基因在荧光假单胞菌FD6防治番茄灰霉病中的功能分析[J]. 园艺学报, 2014, 41(4): 681-686.
[24] 程亮, 游春平, 肖爱萍. 拮抗细菌的研究进展[J]. 江西农业大学学报, 2003, 25(5): 732-737.
[25] 王靖, 苏志芳, 李亚珍, 等. 内生黏质沙雷氏菌LIEH92对向日葵菌核病的防治效果及其防病机制研究[J]. 植物保护, 2016, 42(1): 73-80.
[26] 李霞, 张小平, 李欣. 一株拮抗姜瘟根际青枯假单胞杆菌的沙雷氏菌的分离与鉴定[J]. 湖北农业科学, 2014, 53(20): 4841-4844.
[27] 毕建华, 杨金广, 欧阳明安, 等. 黏质沙雷氏菌次生代谢物对TMV的抑制机理[J]. 中国农业科学, 2014, 47(5): 912-922.
[28] 肖珺, 邹潇潇, 黄惠琴, 等. 海南岛胡椒地穿刺巴斯德芽菌鉴定与多样性分析[J]. 广东农业科学, 2014, 41(17): 134-139.
[29] 钟伏付, 苏娜, 杨廷宪, 等. 魔芋品种选育与改良研究进展[J]. 湖北农业科学, 2011, 50(3): 446-449.
[30] 曹赐生, 王海华. 稻白叶枯菌弱毒株诱导的系统抗性——兼论与防御酶活性的变化[J]. 吉首大学学报(自科版), 2000, 21(2): 58-62.
[31] 吴伟胜, 李玉保, 王守荣, 等. 大肠杆菌噬菌体的研究进展[J]. 江苏农业科学, 2015, 43(8): 8-11.
[32] Fujiwara, A., Fujisawa, M., Hamasaki, R., et al. (2011) Biocontrol of Ralstonia solanacearum by Treatment with Lytic Bacteriophages. Applied & Environmental Microbiology, 77, 4155-4162.
https://doi.org/10.1128/AEM.02847-10
[33] 梁雪杰, 张婷婷, 乔俊卿, 等. 番茄土传病害拮抗菌的筛选、评价及鉴定[J]. 西南农业学报, 2014, 27(3): 1096-1103.
[34] Sunder, A.V., Kumar, A., Naik, N. and Pundle, A.V. (2012) Characterization of a New Bacillus cereus, ATUAVP1846 Strain Producing Penicillin V Acylase, and Optimization of Fermentation Parameters. Annals of Microbiology, 62, 1287-1293.
https://doi.org/10.1007/s13213-011-0374-7
[35] Sellami-Kamoun, A., Ghorbel-Frikha, B., Haddar, A. and Nasri, M. (2011) Enhanced Bacillus cereus, BG1 Protease Production by the Use of Sardinelle (Sardinella aurita) Powder. Annals of Microbiology, 61, 273-280.
https://doi.org/10.1007/s13213-010-0134-0
[36] Yanez-Mendizabal, V., Zeriouh, H., Vinas, I., et al. (2012) Biological Control of Peach Brown Rot ( Monilinia spp.) by Bacillus subtilis CPA-8 Is Based on Production of Fengycin-Like Lipopeptides. European Journal of Plant Pathology, 132, 609-619.
https://doi.org/10.1007/s10658-011-9905-0
[37] 贾丽苑, 贾雨, 黄建新. 拮抗菌应用于微生物杀菌剂的研究现状及展望[J]. 安徽农学通报, 2012, 18(10): 49-52.
[38] 杨蕾, 周国英, 梁军. 2种生防菌株对杨树溃疡病原葡萄座腔菌的抑制作用[J]. 林业科学, 2015, 51(8): 67-73.
[39] Timmusk, S., Grantcharova, N. and Wagner, E.G. (2005) Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms. Applied & Environmental Microbiology, 71, 7292-7300.
https://doi.org/10.1128/AEM.71.11.7292-7300.2005
[40] Shoda, M. (2000) Bacterial Control of Plant Diseases. Journal of Bioscience & Bioengineering, 89, 515-521.
https://doi.org/10.1016/S1389-1723(00)80049-3
[41] 赵玉华, 周蕊, 李俊州, 等. 内生细菌EBS05对烟草诱导抗性的信号转导途径研究[J]. 植物病理学报, 2014, 44(5): 527-535.
[42] 梁艳琼, 唐文, 吴伟怀, 等. 生防菌TB2对甘蔗叶片抗病相关酶活的诱导作用[J]. 福建农业学报, 2016, 31(6): 620-625.
[43] Foldes, T., Banhegyi, I., Herpai, Z., Varga, L. and Szigeti, J. (2000) Isolation of Bacillus Strains from the Rhizosphere of Cereals and in Vitro Screening for Antagonism against Phytopathogenic, Food-Borne Pathogenic and Spoilage Micro-Organisms. Journal of Applied Microbiology, 89, 840-846.
https://doi.org/10.1046/j.1365-2672.2000.01184.x
[44] 李金云, 王慧敏, 王建辉. 根癌病生防菌E26菌株产生细菌素的初步研究[J]. 中国农业科学, 2004, 37(12): 1860- 1865.
[45] Ashwini, N. and Srividya, S. (2014) Potentiality of Bacillus subtilis as Biocontrol Agent for Management of Anthracnose Disease of Chilli Caused by Colletotrichum gloeosporioides OGC1. 3 Biotech, 4, 127-136.
https://doi.org/10.1007/s13205-013-0134-4
[46] Zhao, Y., Selvaraj, J.N., Xing, F., et al. (2014) Antagonistic Action of Bacillus subtilis Strain SG6 on Fusarium graminearum. PLoS ONE, 9, e92486.
https://doi.org/10.1371/journal.pone.0092486
[47] 李晶, 赵晓宇, 张先成, 等. 生防枯草芽孢杆菌B29菌株抗菌蛋白的质谱分析[J]. 黑龙江科学, 2010(2): 29-30.
[48] 余惠荣, 李悦, 李晓菲, 等. 孢短芽孢杆菌B8抑菌物质的理化性质及其分离纯化[J]. 沈阳农业大学学报, 2015, 46(4): 398-403.
[49] 张华, 赵辉, 王鹏涛, 等. 解淀粉芽孢杆菌B10-26抑菌物质的稳定性研究与初步分离纯化[J]. 河南农业科学, 2014, 43(5): 93-96.
[50] 王继良, 杨金库, 冯争光, 等. 枯草芽孢杆菌SYL-6脂肽类化合物的分离与稳定性分析[J]. 现代农业科技, 2015(23): 114-115.
[51] 汤谷月, 刘朝霞, 赵宝梅, 等. 小麦纹枯病菌拮抗菌株X2-3的鉴定及其抗菌特性分析[J]. 山东农业科学, 2016, 48(5): 93-97.
[52] 陈洁梅, 张灿辉, 艾田, 等. 解淀粉芽孢杆菌KN-BL-1及其发酵豆粕产抗菌肽类物质的研究[J]. 中国生物工程杂志, 2014, 34(10): 61-66.
[53] Tamehiro, N., Okamotohosoya, Y., Okamoto, S., et al. (2002) Bacilysocin, a Novel Phospholipid Antibiotic Produced by Bacillus subtilis 168. Antimicrobial Agents & Chemotherapy, 46, 315-320.
https://doi.org/10.1128/AAC.46.2.315-320.2002
[54] 蔡作新, 郑传伟, 田伟波, 等. 氮离子注入诱变选育恩拉霉素高产菌株[J]. 工业微生物, 2015(3): 58-61.
[55] 张鸿, 林志坚, 林赵淼, 等. T-DNA随机插入法获得甘薯蔓割病菌非致病生防菌株[J]. 中国生物防治学报, 2016, 32(5): 610-618.
[56] 顾鹏飞, 吴刚, 胡永红, 等. 低能N+注入选育凝结芽孢杆菌高效生防菌株[J]. 河南农业科学, 2016, 45(5): 87-90.
[57] 甘良, 蓝星杰, 戴蓬博, 等. 放线菌混合菌剂对西瓜枯萎病的防治作用研究[J]. 中国生物防治学报, 2015, 31(4): 516-523.