世界肿瘤研究  >> Vol. 7 No. 3 (July 2017)

循环miR-155与胃癌炎症因子相关性分析及在胃癌诊断中意义研究
Relationship between Circulating miR-155 and Inflammatory Factors in Gastric Carcinoma and Its Significance in the Diagnosis of Gastric Cancer

DOI: 10.12677/WJCR.2017.73011, PDF, HTML, XML, 下载: 1,004  浏览: 1,751 

作者: 洪宏海*, 陈金桃, 林丽英:广州医科大学附属第三医院检验科,广东 广州

关键词: 胃癌miR-155炎症因子Gastric Cancer miR-155 Inflammatory Factor

摘要: 目的:血中miR-155与胃癌炎症因子相关性分析及其在胃癌诊断中意义研究。方法:qRT-PCR检测胃癌病人血清中miR-155相对表达;小分子干扰RNA干扰miR-155后研究其对胃癌细胞株SGC-7901炎症因子分泌作用;ELISA检测炎症因子IL-1β、IL-6、IL-8水平。结果:胃癌病人血清中miR-155水平明显高于健康人血清中miR-155水平,且ROC曲线分析表明血清中miR-155对胃癌诊断具有较好的诊断价值。此外,胃癌病人血清中miR-155水平与炎症因子IL-6、IL-8水平呈正相关;在胃癌细胞中SGC-7901干扰miR-155后明显抑制胃癌细胞SGC-7901炎症因子IL-1β、IL-6、IL-8分泌。结论:血清中miR-155水平对胃癌具有诊断意义,且其调控胃癌炎症因子IL-1β、IL-6、IL-8分泌。
Abstract: Objective: To investigate the correlation between miR-155 and inflammatory factors in gastric cancer and its significance in the diagnosis of gastric cancer. Methods: The relative expression of miR-155 in serum of patients with gastric cancer detected by qRT-PCR; ELISA analysis the secre-tion of inflammatory cytokines including IL-1β, IL-6, IL-8 in gastric cancer cell line SGC-7901, si-lencing miR-155 by small molecule interference. Results: The level of miR-155 in serum of pa-tients with gastric cancer was significantly higher than that in healthy people, and ROC curve analysis showed that the serum miR-155 had a better diagnostic value in the diagnosis of gastric cancer. In addition, inflammatory cytokines IL-6, IL-8 levels were positively correlated with serum miR-155 level in serum of patients with gastric cancer gastric cancer; silencing of miR-155 inhibited inflammatory factor IL-1beta, IL-6, IL-8 secretion in gastric cancer cells. Conclusion: The level of miR-155 in serum is of significance in the diagnosis of gastric cancer, and it can regulate the secretion of inflammatory factors IL-1beta, IL-6 and IL-8 in gastric cancer.

文章引用: 洪宏海, 陈金桃, 林丽英. 循环miR-155与胃癌炎症因子相关性分析及在胃癌诊断中意义研究[J]. 世界肿瘤研究, 2017, 7(3): 69-74. https://doi.org/10.12677/WJCR.2017.73011

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