叶瓜参多肽有机铬及其对糖尿病小鼠辅助降血糖功能的研究

Research of Cucumaria frondosa Polypeptide Organic Chromium and Auxiliary Fall Blood Function on Diabetic Mice

摘要: 目的：研究叶瓜参多肽有机铬对糖尿病小鼠日常饮食、体重及空腹血糖、血清胰岛素水平、糖耐量的影响。方法：采用高糖高脂饲料联合少量多次腹腔注射链脲霉素建立II型糖尿病模型小鼠，随机分为模型对照组、阳性对照组(盐酸二甲基双胍片)、叶瓜参有机铬低、中、高剂量组(5 mg/kg、15 mg/kg、20 mg/kg)，另选10只健康小鼠为正常样品组,连续灌胃35 d，观察记录饮食、体重、空腹血糖值、血清胰岛素和糖耐量变化，根据各项指标探讨叶瓜参多肽有机铬对糖尿病小鼠辅助降血糖的影响。结果：与模型对照组比较，阳性对照组和试验叶瓜参多肽有机铬组都有显著性差异(P < 0.01)，阳性对照组和高剂量叶瓜参多肽有机铬组血糖下降率达到57%和50.7%，血清胰岛素和HOMA-IR分别下降了29.7%、24.7%和32.0%、30.1%；与阳性对照组比较，低、中剂量叶瓜参有机铬都有显著性差异，正常样品组和高剂量叶瓜参多肽有机铬组无显著性差异(P > 0.05)。结论：叶瓜参多肽有机铬对健康正常小鼠无影响，一定量的叶瓜参多肽有机铬对患有糖尿病小鼠的日常饮食、体重、空腹血糖值、血清胰岛素、糖耐量有极显著性影响，能促进糖尿病小鼠的生产发育，能降低小鼠空腹血糖，提高糖耐量的能力，对辅助降血糖功能有显著性作用。

Abstract: Objective: Effect of Cucumaria frondosa polypeptide organic chromium on diet, weight, fasting blood glucose, serum insulin levels and glucose tolerance in diabetic mice. Methods: using high sugar and high fat feed with a small amount of the STZ to built II diabetes model mice by intrape-ritoneal injection. II diabetes model mice were randomly divided into model control group, posi-tive control group, with low, medium and high dose of Cucumaria frondosa polypeptide organic chromium group (5 mg/kg, 15 mg/kg, 20 mg/kg). Choosing 10 healthy mice as normal subjects group to observe and record the changes of diet, weight, fasting blood sugar, serum insulin levels and glucose tolerance with 35 d gavage. According to the indicators, the influence of Cucumaria frondosa polypeptide organic chromium on auxiliary fall blood diabetes mice has been explored. Results: Compared with model control group, positive control group and experimental Cucumaria frondosa polypeptide organic chromium group had significant difference (P < 0.01), positive control group and group with high dose of Cucumaria frondosa polypeptide organic chromium’s rate reached 57% and 57% drop in blood sugar; serum insulin and HOMA IR fell 29.7%, 24.7% and 32.0%, 32.0% respectively. Compared with positive control group, low and medium dose group of Cucumaria frondosa polypeptide organic chromium had significant difference, the normal sample group and high dose group of Cucumaria frondosa polypeptide organic had no significant difference (P > 0.05). Conclusion: Cucumaria frondosa polypeptide organic chromium has no effect on the healthy mice. A certain amount of Cucumaria frondosa polypeptide organic chromium has extremely significant effect on the diet, weight, fasting blood sugar, serum insulin and glucose tolerance on diabetic mice, which can promote the production and development of diabetic mice, reduce fasting blood glucose in mice and improve the ability of glucose tolerance. It has significant effect to auxiliary fall blood sugar function.