免疫细胞功能检测在恶性肿瘤患者外周血中的表达和临床意义

Expression of Immune Cell Function in Peripheral Blood of Patients with Malignant Tumor and Its Clinical Significance

摘要: 目的：通过检测恶性肿瘤患者外周血免疫细胞计数的变化，探讨其在肿瘤患者诊疗中的临床价值。方法：测定60例肿瘤患者，其中食管癌20例，肺癌20例，鼻咽癌10例，其他肿瘤(包括喉癌1例、舌癌1例、乳腺癌3例、皮肤恶性黑色瘤1例、直肠癌4例)10例；及20名正常对照组外周血T淋巴细胞亚群百分比、B淋巴细胞百分比、NK细胞百分比，比较与正常对照组间差异。结果：肿瘤患者组外周血中总T淋巴细胞百分比CD3+、辅助诱导T淋巴细胞百分比CD4+、辅助抑制T淋巴细胞比值CD4+/CD8+与健康组比较明显下降(P < 0.05)；抑制细胞毒T淋巴细胞百分比CD8+与健康组比较略有升高(P > 0.05)。肿瘤患者组总B淋巴细胞百分比、NK细胞百分比与对照组差异无统计学意义。结论：T淋巴细胞亚群是一种检测肿瘤患者免疫功能异常的较好方法，对监测病情和判断预后具有重要意义。

Abstract: Objective: To investigate the clinical value of immune cell in peripheral blood of patients with malignant tumor, and to explore its clinical value in the diagnosis and treatment of cancer patients. Methods: 60 cases of cancer patients, including 20 cases of esophageal cancer, lung cancer in 20 cases, 10 cases of nasopharyngeal carcinoma, other tumors (including laryngeal cancer in 1 case, tongue cancer in 1 case, breast cancer in 3 cases, 1 case of skin malignant melanoma, rectal cancer 4 cases) and 20 normal control group peripheral blood T lymphocyte subsets percentage, B lymphocyte percentage, NK cell percentage, compared with the differences of normal control group. Results: The percentage of total T lymphocytes CD3+, assisted/induced T lymphocyte CD4+ and helped inhibited T lymphocyte ratio CD4+/CD8 in peripheral blood of tumor patients was significantly lower than that of healthy group (P < 0.05). The percentage of inhibition cytotoxicity T lymphocyte CD8+ was slightly higher than that in the healthy group (P > 0.05). There was no significant difference between the percentage of total B lymphocytes and the percentage of NK cells in the tumor group and the control group. Conclusions: T lymphocyte subsets are a good method for detecting immune dysfunction in cancer patients, which are of great significance to monitor the disease and judge the prognosis.