外源激素不同配比对“白草莓”茎尖萌发及增殖的影响研究
Effect of Different Ratio of Exogenous Hormones to the Tip Meristem Generation and Propagation of Pine-Berry
DOI: 10.12677/BR.2017.64029,
PDF, HTML, XML,
被引量
下载:
1,628 浏览:
4,434
作者:
代会会, 康从彬, 吴建豪, 史昊, 李月, 程杰山:鲁东大学农学院农林作物遗传改良中心,山东 烟台
关键词:
白草莓;茎尖培养;外源激素;6-BA浓度梯度;Pine-Berry; Stem Tip Culture; Exogenous Hormones; Concentration Gradients of 6-BA
摘要: 以日本白草莓为试材,研究6-BA浓度梯度及其它激素的配比不同对白草莓茎尖萌发和增殖的影响,结果表明,高浓度6-BA对茎尖萌发有抑制作用,对连续增殖也有不利影响,主要是矮化簇生和畸形;MS培养基加入6-BA 0.5 mg/L和NAA 0.1 mg/L,茎尖生长点萌发时愈伤化严重,且萌发率不高;MS培养基添加6-BA 0.5 mg/L和IBA 0.1 mg/L,“白草莓”茎尖萌发率为99%。第一代增殖MS培养基中添加6-BA 1.5 mg/L,IBA 0.1 mg/L,以后应逐渐降低6-BA浓度,直到降到0.5 mg/L,但IBA浓度保持0.1 mg/L不变,茎尖萌发苗增殖迅速,繁殖系数2.5以上。增殖的白草莓幼苗继代到6-BA 0.5 mg/L和NAA 0.1 mg/L的MS培养基上,长势迅速且健壮。
Abstract:
This paper researches about the effect of different ratio of exogenous hormones to the tip meristem generation and propagation of Japanese pine-berry which was introduced from Japan. 6-BA concentration gradient, hormone ratio, generation and reproduction efficiency were investigated. The results showed that, high concentration of 6-BA can retard the tip stem generation, and has negative effect on propagation. The main effects are dwarf, cluster, and abnormality. On the MS media with 6-BA 0.5 mg/L and NAA 0.1 mg/L, tip stem generating ratio was low and accompanied with callus occurred severely. However, on the MS media with 6-BA 0.5 mg/L and IBA 0.1 mg/L, the generation ratio was 99% in this experiment. First regeneration MS media with 6-BA 1.5 mg/L and IBA 0.1 mg/L, from second regeneration, the 6-BA concentration was gradually decreased to 0.5 mg/L in the end, but the IBA concentration remained unchanged. Under this condition, the generating seedlings regenerated rapidly. The propagation coefficient was higher than 2.5. Propagating seedlings sub-cultured on the MS media with 6-BA 0.5 mg/L and NAA 0.1 mg/L, grew rapidly and healthily.
参考文献
[1]
|
黄小飞. 浅谈丹东地区草莓生产现状及未来发展方向[J]. 农民致富之友, 2014(16): 20.
|
[2]
|
雷家军. 我国草莓生产现状与展望[J]. 中国果树, 2001(1): 49-51.
|
[3]
|
周厚成, 何水涛. 草莓病毒病研究进展[J]. 果树学报, 2003, 20(5): 421-426.
|
[4]
|
何欢乐, 阳静, 蔡润, 潘俊松. 草莓茎尖培养脱毒效果研究[J]. 北方园艺, 2005(5): 79-81.
|
[5]
|
高遐虹, 李梅. 提高草莓茎尖组织培养脱毒效率的研究[J]. 中国果树, 1994(2): 5-6.
|
[6]
|
于丽杰, 崔继哲. 草莓脱病毒苗的诱导及其光合特性研究[J]. 植物研究, 1995, 15(2): 263-268.
|
[7]
|
晁慧娟, 刘敏, 姬谦龙, 等. “甜查理”草莓茎尖培养与快速繁殖研究[J]. 北京农学院学报, 2008, 23(2): 24-27.
|
[8]
|
朴日子, 曹后男, 宗成文. 影响“达赛莱克特”草莓茎尖培养因素的研究[J]. 延边大学农学学报, 2009, 31(3): 184-187.
|
[9]
|
Kozai, T., Kubota, C. and Jeong, B.R. (1997) Environmental Control for the Large Scale Production of Plants through in Vitro Techniques. Plant Cell, Tissue and Organ Culture, 51, 49-56. https://doi.org/10.1023/A:1005809518371
|
[10]
|
李金凤, 糜林, 张计育, 等. 草莓茎尖离体快繁体系研究[J]. 江西农业学报, 2009, 21(6): 46-47.
|
[11]
|
郝文胜, 赵永秀, 赵清辉, 张朝霞. 我国马铃薯茎尖培养脱毒和脱毒试管苗微繁研究进展[J]. 内蒙古农业科技, 2005(增刊): 27-33.
|
[12]
|
刘卫平, 李玉华, 孙秀梅, 王福兴. 马铃薯离体茎尖生长点对几种培养因子的生长反应[J]. 中国马铃薯, 2001(2): 81-82.
|
[13]
|
李晓亮, 张军云, 张钟, 等. 草莓茎尖组织培养和快繁体系的建立[J]. 作物杂志, 2016(4): 68-74.
|