BR  >> Vol. 6 No. 5 (September 2017)

    外源激素对桂北地区原生国兰组培快繁的效应探究
    Explore the Effect of Exogenous Hormones on Rapid Propagation of Tissue Culture of Native Orchids in Northern Guangxi

  • 全文下载: PDF(352KB) HTML   XML   PP.275-281   DOI: 10.12677/BR.2017.65036  
  • 下载量: 161  浏览量: 195   科研立项经费支持

作者:  

郭亮,秦丽凤,文清岚:桂林市林业科学研究所,广西 桂林

关键词:
原生国兰外源激素组培快繁原球茎Native Orchids Exogenous Hormones Tissue Culture and Rapid Propagation Protocorm

摘要:
利用L9(34)正交试验方法,研究IBA、6-BA两种外源激素和活性炭(AC)对桂北地区原生国兰组培快繁的效应。原球茎诱导形成阶段适宜培养基为MS + 6-BA 1.0 mg/L + IBA 0.6 mg/L + AC 4.0 mg/L;原球茎增殖期适宜培养基为MS + 6-BA 1.0 mg/L + IBA 0.4 mg/L + AC 4.0 g/L;原球茎分化期适宜培养基为MS + 6-BA 0.4 mg/L + IBA 0.6 mg/L + AC 0.5 g/L;壮苗及生根阶段适宜培养基为1/2MS + IBA 0.4 mg/L + AC 1.0 g/L。

The effect of IBA and 6-BA two kinds of exogenous hormones and active carbon (AC) on rapid propagation of northern Guangxi native orchid tissue culture was studied by L9 (34) orthogonal test. At the induction stage of protocorms formation, the suitable medium was MS + 6-BA 1.0 mg/L + IBA 0.6 mg/L + AC 4.0 mg/L; at the proliferative stage of protocorms, the suitable medium was MS + 6-BA 1.0 mg/L + IBA 0.4 mg/L + AC 4.0 g/L; in the differentiation stage of protocorms, the suitable medium was MS + 6-BA 0.4 mg/L + IBA 0.6 mg/L + AC 0.5 g/L; in the seedling and rooting stage, the suitable medium was 1/2MS + IBA 0.4 mg/L + AC 1.0 g/L.

文章引用:
郭亮, 秦丽凤, 文清岚. 外源激素对桂北地区原生国兰组培快繁的效应探究[J]. 植物学研究, 2017, 6(5): 275-281. https://doi.org/10.12677/BR.2017.65036

参考文献

[1] 李慧敏. 兰花组培快繁研究进展[J]. 农业研究与应用, 2014(1): 53-56.
[2] 王熊. 兰花快速无性系繁殖的研究及花芽分化的探讨[J]. 植物生理学报, 1984(4): 13-14.
[3] 刘幸佳. 几种国兰组织培养及春兰菌根真俊研究[D]: [硕士学位论文]. 杭州: 浙江农林大学, 2013.
[4] 张先云, 袁秀云, 马杰. 杂交兰的组织培养与快速繁殖技术研究[J]. 河南科学, 2009, 27(4): 419-421.