彩色马铃薯StMyb-like基因克隆表达分析与干涉载体构建

Cloning and Expression Analysis of StMyb-like Gene of Color Potato and Construction of Interference Vector

摘要: 彩色马铃薯中富含花色苷，是天然抗氧化物质来源之一。本研究从彩色马铃薯“黑金刚”中克隆了与花色苷合成相关的调控基因，其编码产物且与马铃薯R2R3-MYB转录因子高度同源，被命名为StMyb-like基因。该基因全长798 bp，编码个265个氨基酸，分子量为30.23 kD。通过半定量RT-PCR分析StMyb-like基因的表达，发现该基因在根和茎中表达量很低，而在富花色苷的薯肉、薯皮、芽和叶片中表达量比较高。为了研究彩色马铃薯StMyb-like基因的功能，利用DNA重组技术构建该基因的植物RNA干涉载体pART-MybRNAi，为后续研究该基因可能所参与的生命活动提供了基础。

Abstract: Color potato is rich in anthocyanins, which is one of the natural antioxidants. In this study, one gene related to anthocyanin synthesis was cloned from the color potato “Black King Kong”, and this gene encoding protein is highly homologous to transcription factor R2R3-MYB, and named as StMyb-like. The whole length of this gene is 798 bp, which encodes a 265 amino acid, with a molecular weight of 30.23 kD. The expression of StMyb-like gene was analyzed by semi quantitative RT-PCR. It was found that the expression level of the gene in roots and stems was very low, but the expression level of anthocyanin in sweet potato, potato skin, buds and leaves was relatively high. In order to study the function of StMyb-like gene in color potato, the pART-MybRNAi interference vector was constructed by using DNA recombination technology, which provides a basis for further study of the possible life activities of the gene.