黄檗叶提取物的体外抗氧化活性研究
Study on Antioxidant Activity in Vitro of Extract from Phellodendron amurense Leaves
DOI: 10.12677/BR.2018.73030, PDF,    国家自然科学基金支持
作者: 王春晶, 张玉红*：东北林业大学森林植物生态学教育部重点实验室，黑龙江 哈尔滨
关键词: 黄檗叶；抗氧化活性；乙醇提取物；DPPH；羟自由基；ABTS；FRAP；Phellodendron amurense Leaves； Antioxidant Activity； Ethanol Extract； DPPH； Hydroxyl Radical； ABTS； FRAP
摘要: 目的：探究黄檗叶醇提物不同极性溶剂提取物的体外抗氧化活性。采用有机溶剂萃取法将黄檗提取物分为石油醚相、乙酸乙酯相、正丁醇相和水相4个不同提取物，通过对1,1-二苯基-2-三硝基苯肼(DPPH)、羟自由基(•OH)、ABTS+•清除能力以及铁离子(Fe3+)还原能力(FRAP法)的测定，比较评价黄檗叶70%醇提取物不同极性组分的抗氧化活性能力。结果表明黄檗叶不同溶剂提取物均具一定的抗氧化活性，并呈现显著剂量效应关系(p < 0.05)；其抗氧化效果因反应体系的不同而不同，且不同溶剂提取物的抗氧化活性有差异。乙酸乙酯相和正丁醇相提取物对DPPH、•OH和ABTS+•的清除能力和对铁离子(Fe3+)还原能力均优于石油醚和水相提取物。黄檗叶乙酸乙酯相和正丁醇相提取物的抗氧化作用较强，是天然抗氧化剂的来源。
Abstract: Aim: To investigate antioxidant activities in vitro of different polarity fractions of extract from ethanol extract of Phellodendron amurense leaves. 4 extractions were obtained in different fractions by using different polarities of solvents such as petroleum ether, ethyl acetate, butanol and water. The antioxidant activity capacity of 4 different polarity fractions from ethanol extract of P. amurense leaves was evaluated by detecting scavenging abilities for 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical, hydroxyl radical (•OH), ABTS+• radical and Ferric Reducing Antioxidant Power method (FRAP) assay. The results showed that the different fractions of extract from P. amurense leaves had revealed antioxidant activity and showed a significant concentration-effect relationship (p < 0.05). The antioxidant effects of 4 fractions from P. amurense leaves all had different antioxidant effects in different reaction systems. There were differences in antioxidant activities among 4 fractions. Extracts of ethyl acetate fraction and butanol fraction had better scavenging effects than petroleum ether fraction and water fraction for DPPH, •OH, ABTS+• and FRAP. The extract from the ethyl acetate and butanol of P. amurense has a strong antioxidant activity and is a source of natural antioxidants.
文章引用：王春晶, 张玉红. 黄檗叶提取物的体外抗氧化活性研究[J]. 植物学研究, 2018, 7(3): 233-240. https://doi.org/10.12677/BR.2018.73030

参考文献

[1] 郭巧生. 药用植物栽培学[J]. 北京: 高等教育出版杜, 2004.
[2] 国家药典委员会. 中华人民共和国药典(一部) [M]. 北京: 化学工业出版社, 2005: 99-100.
[3] Yin, J., Ye, J.P. and Jia, W.P. (2012) Effects and Mechanisms of Berberine in Diabetes Treatment. Acta Pharmaceutica Sinica B, 2, 327-334.
[Google Scholar] [CrossRef] 
[4] 张冠英, 董瑞娟, 廉莲. 川黄柏、关黄柏的化学成分及药理活性研究进展[J]. 沈阳药科大学学报, 2012, 29(10): 812-821.
[5] 张玲, 张东来, 邓晓华. 黄檗雌雄植株增殖培养性别差异研究[J]. 森林工程, 2017, 33(6): 11-14.
[6] Devasagayam, T.P.A., Tilak, J.C., Boloor, K.K., et al. (2004) Free Radicals and Antioxidants in Human Health: Current Status and Future Prospects. Journal of the Association of Physicians of India, 52, 794-804.
[7] Kim, J.W. and Minamikawa, T. (1997) Hydroxyl Radical-Scavenging Effects of Spices and Scavengers from Brown Mustard (Brassica nigra). Bioscience, Biotechnology, and Biochemistry, 61, 118-123.
[Google Scholar] [CrossRef] 
[8] Wang, H., Cao, G. and Prior, R.L. (1996) Total Antioxidant Capacity of Fruits. Journal of Agricultural and Food Chemistry, 44, 701-705.
[Google Scholar] [CrossRef] 
[9] Krishnaiah, D., Sarbatly, R. and Nithyanandam, R. (2011) A Review of the Antioxidant Potential of Medicinal Plant Species. Food and Bioproducts Processing, 89, 217-233.
[Google Scholar] [CrossRef] 
[10] 刘德江, 申健, 田立娟, 等. 野生与栽培蓝靛果果实提取物的体外抗氧化活性[J]. 经济林研究, 2013, 31(3): 103-106.
[11] Li, S., Li, S.K., Gan, R.Y., et al. (2013) Antioxidant Capacities and Total Phenolic Contents of Infusions from 223 Medicinal Plants. Industrial Crops and Products, 51, 289-298.
[Google Scholar] [CrossRef] 
[12] Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science and Technology, 28, 25-30.
[Google Scholar] [CrossRef] 
[13] 胡喜兰, 韩照祥, 陶莹, 等. DPPH•法测定17种植物的抗氧化活性[J]. 食品科技, 2006, 31(10): 264-268.
[14] 陈留勇, 孟宪军, 贾薇, 等. 黄桃水溶性多糖的抗肿瘤作用及清除自由基提高免疫活性研究[J]. 食品科学, 2004, 25(2): 167-170.
[15] 高波. 孔石莼中体外抗氧化活性物质的研究[D]: [硕士学位论文]. 哈尔滨: 哈尔滨工业大学, 2013.
[16] Park, Y.S., Namiesnik, J., Vearasilp, K., et al. (2014) Bioactive Compounds and the Antioxidant Capacity in New Kiwi Fruit Cultivars. Food Chemistry, 165, 354-361.
[Google Scholar] [CrossRef] [PubMed]
[17] Tai, Z., Cai, L., Dai, L., et al. (2011) Antioxidant Activity and Chemical Constituents of Edible Flower of Sophora viciifolia. Food Chemistry, 126, 1648-1654.
[Google Scholar] [CrossRef] [PubMed]
[18] Benzie, I.F.F. and Strain, J.J. (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239, 70-76.
[Google Scholar] [CrossRef] [PubMed]
[19] Duduku, K., Rosalam, S. and Rajesh, N. (2011) A Review of the Antioxidant Potential of Medicinal Plant Species. Food and Bioproducts Processing, 89, 217-233.
[Google Scholar] [CrossRef]