L-抗坏血酸硬脂酸棕榈酸酯包裹氧化铁纳米粒子的磁共振成像以及RAW264.7细胞体外标记检测
Mr Imaging of Superparamagnetic Iron Oxide Nanoparticles Coated L-Ascorbic Acid Stearic Acid Palmitate and in Vitro Labeling of RAW264.7 Cells
DOI: 10.12677/HJBM.2018.82004, PDF,    科研立项经费支持
作者: 赵瑞兰, 李峻峰*：成都理工大学，材料与化学化工学院，四川 成都；王志勇, 刘雨晴：中国科学院，深圳先进技术研究院，广东 深圳
关键词: 超顺磁性氧化铁；RAW264.7；细胞活性；磁共振成像；Superparamagnetic Iron Oxide Nanoparticles； RAW264.7； Cell Viability； Magnetic Resonance Imaging (MRI)
摘要: 目的：应用L-抗坏血酸硬脂酸棕榈酸酯(Vc)包裹的超顺磁性氧化铁纳米粒子(SPIO)，构建纳米探针，考察不同材料组分的磁共振造影剂成像效果，实现RAW264.7小鼠单核巨噬细胞的体外标记并探究其生物活性影响。方法：调节Vc与SPIO质量比例(2:1和5:1)构建纳米Vc-SPIO探针；并完成磁共振成像检测；常规培养RAW264.7巨噬细胞，应用Vc-SPIO纳米探针(铁浓度0~25 ug/ml)完成细胞标记，进行细胞毒性CCK-8检测研究。结果：当Vc:SPIO为2:1时，在实验的铁浓度范围内，细胞依然保持正常活性；当Vc:SPIO为5:1时，随着铁浓度的增大细胞活性降低，当铁浓度大于15 ug/ml时有一定的细胞毒性。结论：Vc-SPIO在低于15 ug/ml浓度时对RAW264.7的生物活性没有明显影响，可以实现RAW264.7细胞标记，为下一步动物实验奠定基础。
Abstract: Objective: The L-ascorbic acid stearic acid palmitate (Vc) had been used to coat superparamagnetic iron oxide nanoparticles (SPIO) to build nano probes. The imaging effects of magnetic resonance imaging agents of different materials were investigated. This contrast probe was applied to label mouse mononuclear macrophage cells-RAW264.7 in vitro. The cellular activity was measured in this study. Methods: The quality ratio of Vc and SPIO were adjusted (2:1 and 5:1) to construct the Vc-SPIO nano probes. And then magnetic resonance imaging was completed. Mice RAW264.7 macrophages were cultured routinely. Using the Vc-SPIO nanometer probe (iron concentration 0~25 ug/ml) to complete the cell marking, the cytotoxicity of cell was detected by CCK-8 test. Results: When the molecular ratio of Vc:SPIO was about 2:1, the cells remained normal activity under the concentration of iron in the experiment. However, if the molecular ratio of Vc:SPIO was about 5:1, cell activity will decrease with the increase of iron concentration. When iron concentration was greater than 15 ug/ml, it exhibited toxicity. Conclusion: Vc-SPIO had no obvious effect on the biological activity of RAW264.7 with the concentration below 15 ug/ml. It could be used to label RAW264.7, which lay the foundation for the next animal experiment.
文章引用：赵瑞兰, 王志勇, 刘雨晴, 李峻峰. L-抗坏血酸硬脂酸棕榈酸酯包裹氧化铁纳米粒子的磁共振成像以及RAW264.7细胞体外标记检测[J]. 生物医学, 2018, 8(2): 27-33. https://doi.org/10.12677/HJBM.2018.82004

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