低温处理后两个品种水仙花瓣和叶片的解剖学研究

Anatomic Study on Petals and Leaves of Two Different Types of Narcissus tazetta var. chinensis after Low Temperature Preservation

摘要: 本实验主要是研究低温保存后两个品种水仙(Narcissus tazetta var. chinensis)叶片和花瓣的解剖学结构。通过低温贮藏的两个品种水仙的三年生鳞茎，其生长开花时间延迟，在非正常开花季节进行常规水培；再利用石蜡切片技术，研究其在幼嫩期、生长期和成熟期三个阶段的花瓣、叶片解剖学结构，与矮壮素处理的材料进行比较。研究发现，经过低温贮藏后，两个品种花瓣和叶片的薄壁组织和维管束在生长期和成熟期的变化与矮壮素处理的材料有差异，薄壁细胞解体提前，花瓣维管束、叶片小维管束出现木质部延伸现象，半周木维管束明显。研究低温贮藏对水仙花瓣与叶片的解剖学结构的影响，能为进一步应用生长延缓剂于水仙上以改变和延长水仙花期提供理论依据。

Abstract: This experiment focuses on the anatomic structures of the petals and leaves of two different types of Narcissus tazetta var. chinensis after low temperature preservation. After their bulbs of three-year growths were preserved at low temperatures, they had their growth and bloom delayed. They were cultivated in water as normal, and their petals and leaves in three different periods, namely tender period, growth period and mature period, were then studied by means of paraffin sectioning method. Their anatomic structure was then compared with that of the paclobatrazol inhibited types. The study shows that the parenchyma tissue and vascular bundle of their petals and leaves change differently in the growth period and the mature period when compared with those of the paclobatrazol inhibited types. That is, the cells in the parenchyma tissue disintegrated earlier, while the vascular bundles in the petals and small vascular bundles in the leaves stretched in the xylem, with clear semi-amphivasal vascular bundles. Studies on the effects of low temperature preservation on the anatomic structure of narcissus petals and leaves can offer theoretic basis for the further use of growth retardant on narcissus, which may change and lengthen the flowering period.