13CO2脉冲标记植物的方法及效果研究

Method and Effects of 13CO2 Pulsed Labelling Plant

摘要: 为了探讨植物13C标记的方法及其效果，采用13CO2脉冲标记法对湿地植物及由其垦殖而来的旱田主栽作物大豆进行了标记。结果显示：经过四次标记，湿地植物和大豆植株δ13C值分别从−16.43‰和−28.33‰增加到1185.62‰和1311.82‰；Atom%13C分别比未标记的湿地植物和大豆植株高1.198倍和1.34倍，湿地植物根、茎和叶的Atom%13C比未标记增加了1.03、1.78和0.91倍，大豆根、茎和叶的Atom%13C比未标记增加了1.20、2.19和2.99倍，大豆较湿地植物标记效果更好。湿地植物和大豆Atom%13C呈现地上部分高于地下部分的趋势，大豆更明显。随标记次数(生育期)增加，湿地植物根Atom%13C先增加后降低，茎逐渐增加，叶先降低后增加；大豆根和茎Atom%13C也是先增加后降低，叶逐渐增加，同种植物各器官在不同生育期13C情况不同。13CO2脉冲标记法标记湿地植物和大豆效果良好，简单易行，实用可靠。同种标记方法和标记条件下，不同植物标记效果有差异，同类植物不同器官标记效果不同，同类植物相同器官不同标记时间的标记效果亦有不同，据此可根据需求调整标记原材料用量、标记时间和标记频率等，达到标记目的。

Abstract: The 13CO2 pulse labeling method was used to label the wetland plants and soybeans grown in wet-land and soybean field, the soybean field was reclaimed from wetland. The results showed that the δ13C values of wetland plants and soybeans increased from −16.43‰ and −28.33‰ to 1185.62‰ and 1311.82‰ respectively, after four labels. Atom%13C was 1.198 times and 1.34 times higher than unlabeled wetland plants and soybeans, respectively. And Atom%13C in roots, stems, and leaves of wetland plants increased by 1.03, 1.78, and 0.91 times compared with unlabeled, At-om%13C in soybeans roots, stems, and leaves increased by 1.20, 2.19, and 2.99 times compared to unlabeled; soybeans have better labeling effect than wetland plants. Wetland plants and soybeans Atom%13C showed a trend that the aboveground-part was higher than the underground-part, and the soybeans were more pronounced. With the increase of labeling times (growth period), the roots Atom%13C of wetland plants first increased and then decreased, stems gradually increased and the leaves first decreased and then increased. Soybeans roots and stems Atom%13C also increased first and then decreased, leaves gradually increased. 13C situation in every organs of the same plants in different growth periods is different. The 13CO2 pulse labeling method is effective for the labeling of wetland plants and soybeans. It is simple, practical, and reliable. Under the same labeling methods and labeling conditions, labeling effects are different for different plants, the labeling effects for different organs in same plants are different, the labeling effects of the same organs in the same species of plants are also different with different labeling time. According to this study, the quantity of raw material (13CO2) dosage, labeling time and labeling frequency can be adjusted to achieve the labelling purpose.