黄瓜抗坏血酸过氧化物酶基因的鉴定和特征分析
Identification and Characterization of APX Genes in Cucumber
DOI: 10.12677/HJCB.2018.82005, PDF,  被引量    科研立项经费支持
作者: 李利斌, 孟昭娟, 王永强, 杨桂兰, 杨宗辉, 曹齐卫*：山东省农业科学院蔬菜花卉研究所;国家蔬菜改良中心山东分中心;农业部黄淮地区蔬菜科学观测实验站(山东);山东省设施蔬菜生物学重点实验室,山东 济南；纪复勤, 韩梅香：山东宁阳万丰种业有限公司,山东 宁阳
关键词: 黄瓜；抗坏血酸过氧化物酶基因；结构；系统进化；顺式元件；Cucumis sativus L.； Ascorbate Peroxidase Gene； Structure； Phylogeny； Cis-Element
摘要: 植物抗坏血酸过氧化物酶基因在逆境应答和发育过程中具有重要功能，但是在我国重要蔬菜黄瓜中缺乏报道。本文通过生物信息学的方法，从黄瓜基因组中鉴定出6个抗血酸过氧化物酶基因，并对它们的染色体分布、基因结构、系统进化和顺式元件进行了系统分析。结果显示，黄瓜APX分布在不同的染色体上，编码区大小在750~1425 bp，含有8个以上外显子，预测编码蛋白定位在细胞质或者叶绿体中。它们都具有抗坏血酸过氧化物酶的特征结构域和磷酸化及豆蔻酰化位点。在它们的上游基因序列中，存在多个逆境和激素应答顺式调控元件，暗示它们在逆境应答中具有一定功能。
Abstract: Plant ascorbate peroxidase (APX) genes play significant roles in environmental stress response and development. However, the knowledge about them in the important vegetable cucumber is scarce. In this paper, 6 APX genes in cucumber genome were identified through bioinformatics method. And their chromosome location, gene exon-intron structure, phylogeny, and cis-elements were systematically analyzed. The results showed that cucumber APX genes distribute in different chromosomes, and their putative coding sequence size ranges from 750 bp to 1425 bp. They all have 8 or more exons, and their putative encoded proteins are predicted to localize in cytosol or chloroplast. There are typical ascorbate peroxidase domain, myristoylation sites, and phospharylation sites in APXs encoded proteins of cucumber. And also, multiple cis-elements re-sponsive to different environmental stimuli and hormones are existed in cucumber APX upstream sequences, which suggests they may have some roles in environmental stress responses.
文章引用：李利斌, 孟昭娟, 王永强, 杨桂兰, 杨宗辉, 纪复勤, 韩梅香, 曹齐卫. 黄瓜抗坏血酸过氧化物酶基因的鉴定和特征分析[J]. 计算生物学, 2018, 8(2): 33-39. https://doi.org/10.12677/HJCB.2018.82005

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