紫杉醇联合TLR7/8激动剂3M-052诱导小鼠结肠癌CT26细胞凋亡的研究

Paclitaxel Combined with TLR7/8 Agonist 3M-052 Inducing the Apoptosis of Mouse Colon Cancer CT26 Cells

摘要: 目的：探讨紫杉醇联合Toll样受体7/8激动剂3M-052诱导小鼠结肠癌CT26细胞凋亡的作用及其机制。方法：使用不同浓度的3M-052和PTX联合处理CT26细胞，24 h后检测细胞活力，分析PTX与3M-052协同作用的浓度；并采用Western blot法检测CT26细胞中的Bax、Bcl-2、active-Caspase3以及cleaved-PARP I的表达情况。结果：3M-052可以明显上调小鼠PBMC分泌的TNF-α；3M-052与PTX单药或联用时均可上调Bax/Bcl-2比例，促进Caspase3活化，增强PARP I蛋白剪切，最终导致细胞凋亡。结论：3M-052或PTX单独使用均可促进CT26细胞凋亡，抑制其增殖，联用时效果更佳。

Abstract: Objective: To investigate the effect and mechanism of paclitaxel combined with Toll-like receptor 7/8 agonist 3M-052 on the apoptosis of mouse colon cancer CT26 cells. Methods: CT26 cells were treated with different concentrations of 3M-052 and PTX. The cell viability was measured after 24 h, and the synergistic concentration of PTX and 3M-052 was analyzed. The expression of Bax, Bcl-2, active-Caspase3 and cleaved-PARP I in CT26 cells were detected by Western blot. Results: 3M-052 could induce TNF-α production in mouse PBMC. Exclusive or combined use of 3M-052 and PTX could up-regulate the proportion of Bax/Bcl-2 in cells, promote the shearing of Caspase3 and PARP I protein, therefore induce cell apoptosis. Conclusion: Both 3M-052 and PTX can promote the apoptosis of CT26 cells, and inhibit its proliferation, while the effect would be better when combined.