芪冬颐心口服液治疗儿童病毒性心肌炎的临床效果及机制研究

The Curative Effects of Qidong Yixin Oral Solution on Infantile Viral Myocarditis

摘要: 目的：观察芪冬颐心口服液对儿童病毒性心肌炎的临床效果，并建立心肌炎大鼠模型探讨其可能作用机制。方法：将本院2012年6月~2017年6月收治的56例儿童病毒性心肌炎患者随机分为两组，观察组和对照组，每组28例，两组均给予常规治疗，观察组在此基础上采用芪冬颐心口服液治疗，比较两组疗效、心电图、心肌酶及血清中IL-2、IL-6水平的变化。另外用阿霉素制造大鼠心肌炎模型，HE染色观察经芪冬颐心口服液干预后大鼠心肌细胞形态学的变化，western blotting检测其MMP-2蛋白的表达。结果：观察组疗效和心电图总有效率显著高于对照组(p < 0.05)，肌酸激酶与乳酸脱氢酶显著低于对照组(p < 0.01)，血清IL-2和IL-6水平显著低于对照组(p < 0.01)；另，芪冬颐心口服液能显著改善心肌炎大鼠心肌细胞的形态，抑制MMP-2蛋白的表达。结论：芪冬颐心口服液对病毒性心肌炎儿童及大鼠可能有一定治疗作用，其机制可能与抑制MMP-2蛋白的表达有关。

Abstract: Objective: To observe the clinical effect of Qidong Yixin oral liquid on children with viral myocarditis and establish a rat model of myocarditis to explore its possible mechanism. Methods: 56 cases of children with viral myocarditis treated in our hospital in June 2012-2017 were randomly divided into two groups, the observation group and the control group, 28 cases in each group, and the two groups were given routine treatment. The observation group was treated with Qidong Yixin oral liquid on this basis, and compared the curative effect, electrocardiogram, myocardial enzyme and serum IL-2 and IL-6 in the two groups. In addition, rat myocarditis model was made with adriamycin and HE staining was used to observe the morphological changes of rat cardiac myocytes after the intervention of Qidong Yixin oral liquid. The expression of MMP-2 protein was detected by Western blotting. Results: The total effective rate of the observation group and the electrocardiogram was significantly higher than that of the control group (p < 0.05). The creatine kinase and lactate dehydrogenase were significantly lower than those of the control group (p < 0.01), the serum level of IL-2 and IL-6 was significantly lower than that of the control group (p < 0.01), and the Qi Dong heart oral liquid could significantly improve the morphology of cardiac my-ocytes in the myocarditis and inhibit the expression of MMP-2 protein. Conclusion: Qidong Yixin oral liquid may have some therapeutic effects on children with viral myocarditis and rats, and its mechanism may be related to inhibiting the expression of MMP-2 protein.